Design, Synthesis, Characterization and Biological Evaluation of Iron Oxide Magnetic Nanoparticles Coated with Chitosan/Alginate Polymer

Subject Areas : Journal of Animal Biology

Elham Rostami ¹ (Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran)
Elham Hovazi ² (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran)

Received: 2022-08-15 Accepted : 2022-11-06 Published : 2023-08-23

Keywords: Chitosan, Iron Oxide, Magnetic nanoparticles, Alginate, biopolymer,

Abstract :

In recent years, the use of nanoparticles in diagnosis, drug delivery and treatment has received much attention due to the small size of these particles and the increase in the surface-to-volume ratio. The most important problem when treating cancer with chemotherapy is the lack of access to the central parts of the mass due to its less blood supply. The purpose of this research was to investigate the toxicity of iron oxide nanoparticles coated with chitosan/alginate biopolymer on melanoma cancer cells of Hep G2 cells. In this research, magnetic iron nanoparticles were coated with two biopolymers, chitosan and alginate. The size and morphology of the surface of these nanoparticles were checked by size measuring device and scanning electron microscope, also the binding of functional groups of chitosan and alginate to magnetic iron nanoparticles was checked by infrared spectrometer. It was treated using magnetic iron nanoparticles and modified nanoparticles for 24 hours and the IC50 concentration of the compounds was estimated. The toxic properties of these nanoparticles were evaluated by MTT test and acridine orange/ethidium bromide staining. Examining the photos of the scanning electron microscope and the size measuring device showed a size of 50 nm for the modified iron nanoparticles. The shape of these nanoparticles was observed to be completely round and spherical. Biological investigations of nanoparticles definitely confirmed the effective coating of nanoparticles by chitosan and alginate biopolymers. According to the findings, magnetic iron nanoparticles had higher toxic effects depending on the concentration and their IC50 concentration was about 134 μM/ml, while the coated nanoparticles had significantly lower toxic effects and at concentrations below 25 μM/ml, they had no significant toxicity on Hep G2 cells. .

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