The Effect of Bornyl Acetate on Male Alzheimer's Disease Wistar Rats and In Vitro Amyloid Fibrils and Investigating the Immobility Stress

Subject Areas : Journal of Animal Biology

Hamid-Reza Alipour ¹ , Parichehreh Yaghmaei ² , Shahin Ahmadian ³ , Maryam Ghobeh ⁴ , Azadeh Ebrahim-Habibi ⁵

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
3 - Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Received: 2022-02-14 Accepted : 2022-02-19 Published : 2022-11-22

Keywords: Memory, bornyl acetate, immobility stress, Amyloid Plaques, Aβ42,

Abstract :

Alzheimer's disease is a neurodegenerative disorder that results in neuronal death and memory loss as it progresses. Following the progression of this disease, the production of free radicals and inflammatory factors occurs and it is now increasingly widespread, especially among the elderly. Studies show that Bornyl acetate, as a monoterpene, has antioxidant and anti-inflammatory effects. Therefore, the present study aims at assessing the effects of Bornyl acetate on AD male Wistar rats. Aβ1-42 was injected into the hippocampus of male rats bilaterally to create the Alzheimer's model. The therapeutic as well as the preventive effects of Bornyl acetate at a dosage (50, 20 mg/kg) were also investigated. Moreover, biochemical enzymes, SOD and MDA, shuttle box behavioral test, histological studies and hematoxylin & eosin and thioflavin-S staining were used to evaluate neurogenesis and amyloid plaques and the effects of short-term restraint stress on AD rats as well as the effects of the compound in vitro. Biochemical factors, histological images and behavioral test of the AD group were significantly different from the control groups and AD group showed a significant decline in learning and memory indices compared with the control group. Also, receiving a Bornyl acetate caused a significant increase in neurogenesis and a significant decrease in plaque density as well as significant changes in biochemical indices and behavioral tests. In addition, Bornyl acetate + restraint stress (R) group elevated learning speed and improved memory compared with the AD group. Notably, the above combination reduced amyloid fibrils in vitro. It seems that this compound can improve memory disorders and complications according to the results of the present study and previous reports suggest the anti-inflammatory and antioxidant effects of Bornyl acetate. 

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