Assessment of Human In-vitro Full Thickness Skin Graft Viability and the Use of Gentamicin Antibiotic on This Process

Subject Areas : Journal of Animal Biology

Marjan Mohamadali ¹ , Ali Ghiaseddin ^{2 *} , Shiva Irani ³ , Mohammad Amir Amirkhani ⁴ , Mostafa Dahmardehei ⁵

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Institute for Stem Cell Research and Regenerative Medicine, Tehran University of Medical Science, Tehran, Iran |Department of Anatomical Sciences, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Skin and Stem Cell Research Center, Tehran University of Medical Science, Tehran, Iran
5 - Department of Plastic and Reconstructive Surgery, Burn Research Center, Iran University of Medical Sciences, Tehran, Iran

Received: 2023-03-01 Accepted : 2023-08-20 Published : 2024-02-20

Keywords: viability, Gentamicin, bacterial contamination, Human skin graft,

Abstract :

Nowadays, skin grafts use to replace lost or damaged skin around the world. Natural contaminants can exist on the skin and this contamination can be a threat to graft recipients. In this study, after obtaining human full thickness skin graft sample and culturing it in-vitro condition, skin samples were evaluated for cell viability and the presence of bacterial contamination during 72 hr. The amount of microbial contamination in the presence and absence of antibiotic gentamicin 0.02% was also investigated by standard methods. High cell viability during 72 hr in normal skin tissue samples was confirmed by the acridine orange staining. Electron scanning microscope observations, hematoxylin-eosin, and Masson's trichrome staining images clearly showed the difference between the two experimental groups. Skin graft samples lost the layers of dermis and epidermis (except the stratum corneum layer), in the absence of gentamicin due to contamination with bacteria which can be seen in the obtained images. Finally, the obtained results clearly showed the effect of high microbial contamination on the skin tissue structure, which can be caused by various factors.

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