Investigating the Effect of Cinnamaldehyde on the Changes of Bax, BCL2, TGF-β Genes Expression in Cancer Cells of Rat’s Gastric Tissue by Dimethylhydrazine

Subject Areas : Journal of Animal Biology

Saber Gharatapehloo ¹ , Tahereh Naji ^{2 *} , Abdolreza Mohammadnia ³

1 - Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences Unit, Islamic Azad University, Tehran, Iran
2 - Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences Unit, Islamic Azad University, Tehran, Iran
3 - Chronic Respiratory Diseases Research Center, Tuberculosis and Lung Diseases Research Institute, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Received: 2022-01-15 Accepted : 2022-06-12 Published : 2022-11-22

Keywords: gastric cancer, Cinnamaldehyde, Dimethylhydrazine, Gene expression changes,

Abstract :

The studies showed Bax and Bcl2, as very important genes in the process of apoptosis that can prevent cancer. The role of TGFβ gene has been also proven as a very important gene in the process of invasion and metastasis in cancers. This study aims at evaluating the expression changes of these three very important genes in cancer cells of rat gastric tissue by Dimethylhydrazine. In this study, forty male Wistar rats and 53 weeks old rats were evaluated and were divided into four ten-groups. The control group received only water and food, the second group received carcinogens without Cinnamaldehyde, the third group received only Cinnamaldehyde and the fourth group received carcinogens with Cinnamaldehyde at the same time. Then, the gastric tissue of rats was isolated and evaluated for the expression of Bax, Bcl2 and TGFβ genes with real-time PCR. The data was analyzed with one-way Anova using SPSS. The results of statistical analysis showed that the expression of in the carcinogen receptor Cinnamaldehyde increased in Bax, decreased in Bcl2 but increased in TGFβ genes compared with the control group, respectively. The results of this study showed that Cinnamaldehyde can be altered by changes in the expression of genes and it is hoped that in further researches, Cinnamaldehyde be considered as a candidate for therapeutic supplements.

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