The Apoptotic Effects of Saccharomyces cerevisiae Cytoplasmic Extract on MCF-7 Cell Line

Subject Areas : Journal of Animal Biology

Ahmadreza Gholamian ¹ , Mohammad Heidarpour ² , Hadi MohebAlian ³

1 - Department of BasicScience, Faculty of Veterinary Medicine, Ferdowsi University, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University, Iran
3 - Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University, Iran

Received: 2023-04-08 Accepted : 2023-05-17 Published : 2023-11-22

Keywords: breast cancer, MTT Assay, Saccharomyces cerevisiae, Flow Cytometry,

Abstract :

Although many studies have been conducted on the anticancer effects of plant extracts, few studies have been done on the anticancer effects of fungal extracts. The aim of this study was to investigate the apoptotic effects of Saccharomyces cerevisiae cytoplasmic extract on MCF-7 cell line. In this experimental laboratory study, MCF-7 breast cancer cells were treated with 500, 1000, and 2000 µg/mL of Saccharomyces cerevisiae extract for 24, 48, and 72 hours. Cell viability was assessed using MTT assay and cellular apoptosis was determine using flow cytometry. The data were analyzed using one-way analysis of variance. The results of the MTT test showed the percentage of cell viability decreased corresponding to an increase in the concentration and the treatment duration of the extract such that the lowest percentage of cell viability was observed at a concentration of 2000 and a duration of 72 hours. The flow cytometry results confirmed apoptotic cell death in cancer cells. The results of this study indicate the cytotoxic effects of Saccharomyces cerevisiae extract on breast cancer cells. The flow cytometry results confirmed that the cytotoxic effects were due to the induction of apoptosis in cancer cells.

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