The Effect of an Aerobic Activity Course in Reducing the Side Effects of Anabolic Steroids with Different Dosages on some Biochemical and Histopathological Indicators of Heart Tissue in Male Wistar Rats

Subject Areas : Journal of Animal Biology

Iman Mazji ¹ (1. Department of Physical Education and Sports Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran.)
Ebrahim SHEKH-NAZARI ² (Department of Physical Education and Sports Sciences, Ayat Amoly Branch, Islamic Azad University, Amoly, Iran)
Asieh Abbasi-daloii ³ (Department of Physical Education and Sports Sciences, Ayat Amoly Branch, Islamic Azad University, Amoly, Iran)
Sayyed-Javad Ziaolhagh ⁴ (Department of Physical Education and Sports Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran.)

Received: 2021-12-06 Accepted : 2022-03-22 Published : 2022-11-22

Keywords: Testosterone, Aerobic exercise, Cardiac Troponin, Boldenone, Anabolic Steroids,

Abstract :

In addition to their therapeutic purposes, steroids have been abused a lot and have had many side effects. Therefore, this study aims at investigating the effect of eight weeks of aerobic exercise on structural and biochemical changes in the heart tissue of male Wistar rats receiving therapeutic dosages and abuse of Boldenone steroid. Therefore, 49 male rats with 12 weeks of age and 190 g mean weight were divided into 7 groups: control group, sham, therapeutic dosage (2 mg / kg), abuse dosage (5 mg / kg), aerobic exercise, aerobic exercise + therapeutic dosage and aerobic exercise + abuse dosage. Injections were given in the same day of the week on the back of rats with a graduated insulin syringe and placebo was used. The aerobic protocol included running five days a week for eight weeks on a rodent treadmill at a speed of 30 meters per minute and 60 minutes training. The samples were first anesthetized and blood and tissue samples were transferred to the relevant laboratories. The results were also analyzed by SPSS software and one-way analysis of variance and Bonferroni post hoc test with a significance level of 0.05. The results showed that there was no significant difference between the groups in final weight gain and cardiac troponin (p <0.05). Also, plasma testosterone levels in steroid-receiving rats decreased significantly in both therapeutic and abusive dosages compared to the exercise groups (p <0.05). On the other hand, the amount of running distance in rodent aerobic function test in steroid and exercise groups was significantly higher than other groups (p <0.05). Generally, data showed that aerobic exercise can prevent the reduction of endogenous testosterone but it has no effect on cardiac troponin levels and rat weight. In addition, Boldenone steroid appears to be effective on increasing the aerobic function of rats.

References:

1. Abdelhafez H.M. 2014. Histological, histochemical and ultrastructural studies on the effect of Deca-Durabolin and whey protein isolate on cardiac muscle in adult male albino rats. International Journal of Advanced Research, 2(10):164-187.


2. Ahmadi M., Abbassi-Daloii A., Ziaolhagh S.J., Yahyaei B. 2018. Structural changes of cardiac tissue in response to boldenone supplementation with or without alcoholic extract of jujuba fruit during resistance training in male Wistar rats. Feyz Journal of Kashan University of Medical Sciences, 21(6): 534-542.


3. Alrabadi N., Jarrah M.I., Alzoubi K.H. 2020. Acute myocardial infarction with cardiogenic shock in a young physically active physician concurrently using the anabolic steroid sustanon: A case report. Biomedical Reports, 13(3): 1-10.


4. Andrews M.A., Magee C.D., Combest T.M., Allard R.J., Douglas K.M. 2018. Physical effects of anabolic-androgenic steroids in healthy exercising adults: A systematic review and meta-analysis. Current Sports Medicine Reports, 17(7): 232-241.


5. Ang E., Mweempwa A., Heron C., Ahn Y., Rivalland G., Ha L.Y., Deva S. 2021. Cardiac Troponin I and T in Checkpoint Inhibitor–associated Myositis and Myocarditis. Journal of Immunotherapy, 44(4): 162-163.


6. Bates G., Begley E., Tod D., Jones L., Leavey C., McVeigh J. 2019. A systematic review investigating the behaviour change strategies in interventions to prevent misuse of anabolic steroids. Journal of Health Psychology, 24(11): 1595-1612.


7. Behairy A., El-Sharkawy N.I., Saber T.M., Soliman M.M., Metwally M.M.M.., Abd El-Rahman GI., Abd-Elhakim Y.M., El Deib M.M. 2020. The modulatory role of vitamin C in boldenone undecylenate induced testicular oxidative damage and androgen receptor dysregulation in adult male rats. Antioxidants, 9(11): 1053.


8. Bornstein A.B., Rao S.S, Marwaha K. 2021. Left Ventricular Hypertrophy. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan. Available from:https://www.ncbi.nlm.nih.gov/books/NBK557534/.


9. Christou M.A., Christou P.A., Markozannes G., Tsatsoulis A., Mastorakos G., Tigas S. 2017. Effects of anabolic androgenic steroids on the reproductive system of athletes and recreational users: a systematic review and meta-analysis. Sports Medicine, 47(9): 1869-1883.


10. Habibpoor Karimabadi F. 2018. Evaluation of ziziphus jujube extract effect during endurance training on cardiac tissue in wistar male rats toxicated by boldenone. Journal of Knowledge and Health in Basic Medical Sciences, 13(2): 42-9.


11. Hernández-Guerra A.I., Tapia J., Menéndez-Quintanal L.M., Lucena J.S. 2019. Sudden cardiac death in anabolic androgenic steroids abuse: Case report and literature review. Forensic sciences research, 4(3): 267-273.


12. Hoseini M., Yousefi B., Khazaei A.A. 2020. The Prevalence of Anabolic-Androgenic Steroids Abuse, Knowledge and Attitude of Their Side Effects, and Attitude Toward Them Among the Female Bodybuilding Athletes in Kermanshah. Journal of Fasa University of Medical Sciences, 10(3): 2439-2447.


13. Wang J., Liew O.W., Richards A.M., Chen Y.T. 2016. Overview of MicroRNAs in Cardiac Hypertrophy, Fibrosis, and Apoptosis. International Journal of Molecular Sciences, 17(5):749.


14. Jones T.H., Kelly D.M. 2018. Randomized controlled trials–mechanistic studies of testosterone and the cardiovascular system. Asian Journal of Andrology, 20(2): 120.


15. Joyce W., Wang T. 2021. How cardiac output is regulated: August Krogh's proto-Guytonian understanding of the importance of venous return. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 253: 110861.


16. Oliel J. 2019. Aerobic Exercise vs. Pharmacology in Adults with Attention Deficit Hyperactivity Disorder (ADHD): Pilot Study. MSc Thesis, Department of Psychiatry, McGill University, Canada.


17. Patanè F.G., Liberto A., Maria Maglitto A.N., Malandrino P., Esposito M., Amico F., Cocimano G., Rosi G.L., Condorelli D.., Nunno N.D., Montana A. 2020. Nandrolone decanoate: use, abuse and side effects. Medicina, 56(11): 606.


18. Reyes-Vallejo L. 2020. Current use and abuse of anabolic steroids. Actas Urológicas Españolas (English Edition), 44(5): 309-313.


19. Saber T.M., Omran B.H.F., El Deib M.M., El-Sharkawy N.I., Metwally M.M.M., Abd-Elhakim Y.M. 2021. Early postmortem biochemical, histological, and immunohistochemical alterations in skeletal muscles of rats exposed to boldenone undecylenate: Forensic implication. Journal of Forensic and Legal Medicine, 83: 102248.


20. Spence S., French A., Penderis J., Macfarlane L., Gutierrez-Quintana R., Dickson L., Holmes K., McLauchlan G. 2019. The occurrence of cardiac abnormalities in canine steroid responsive meningitis arteritis. Journal of Small Animal Practice, 60(4): 204-211.


21. Tousson E. 2016. Histopathological alterations after a growth promoter boldenone injection in rabbits. Toxicology and Industrial Health, 32(2): 299-305.


22. Tung Y.T., Hsu Y.J., Liao C.C., Ho S.T., Huang C.C., Huang W.C. 2019. Physiological and biochemical effects of intrinsically high and low exercise capacities through multiomics approaches. Frontiers in Physiology, 10: 1201.


23. Van der Windt D.J., Sud V., Zhang H., Tsung A., Huang H. 2018. The effects of physical exercise on fatty liver disease. Gene Expression, 18(2): 89.


24. Ziaolhagh S.J., Khojasteh L., Ziaolhagh S.S., Yahyaei B. 2018. The effect of boldenone anabolic steroid, and endurance and resistance training on liver damage markers in rats. Feyz Journal of Kashan University of Medical Sciences, 22(2): 143-152.