Investigating The Effects of Trans-chalcone on Insulin Amyloid Fibrillation

Subject Areas : Journal of Animal Biology

Maria Omidi Shahsavandi ¹ , Parichehr Yaghmaee ² , Shahin Ahmadian ³ , Azadeh Ebrahim-Habibi ⁴

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Tehran Biochemistry and Biophysics Research Center, University of Tehran, Tehran, Iran
4 - Endocrine and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran

Received: 2023-06-17 Accepted : 2023-07-31 Published : 2024-02-20

Keywords: insulin, Aromatic compounds, docking, Congo Red, Trans-chalcone, Amyloid aggregates, Thioflavin S,

Abstract :

Amyloid fibrils are string-like aggregates that form due to the disruption of natural structure and misfolding of various types of peptides and proteins. These amyloid aggregates are associated with some neurodegenerative and systemic diseases such as Alzheimer's, Parkinson's, and type 2 diabetes. The aim of this study was to investigate the inhibitory effects of Trans-chalcone on insulin fibrillation (as a model protein). In this study, insulin was incubated for 24 hours in the presence and absence of Trans-chalcone under amyloid formation conditions. The formed fibrils were analyzed using various techniques including Congo red absorption spectral changes by spectroscopy, electron microscopy images by TEM, and fluorescent staining of amyloid aggregates by fluorescent microscopy. Then, possible interactions between Trans-chalcone and insulin were analyzed using molecular docking method by AutoDock software. The results showed a significant reduction in amyloid fibril formation in the additive-containing sample compared to the control sample. This study provides evidence that Trans-chalcone has an inhibitory effect on insulin fibrillation and could be a potential therapeutic agent for amyloid-related diseases.

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