Investigating the combined effect of endostatin along with endometrial stem cells expressing TSP-1 anti-angiogenic gene on angiogenic induce factors on the treatment of prostate cancer in rats
Subject Areas : Journal of Animal Biology
hossein Attar shakeri
1
(Department of Animal Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran)
Esmail Fattahi
2
(Department of Animal Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran)
Mohammadreza Nowroozi
3
(Department of Animal Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran)
Seyed Ali Momeni
4
(Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran)
Abbas Piryai
5
(Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran)
Keywords: Rat, prostate cancer, endometrial stem cells, Endostatin, Anti-angiogenesis,
Abstract :
Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎have gained attention as adjuvants for this cancer. Here, we report for the first time that ‎endostatin along with endometrial stem cells expressing TSP-1 anti-angiogenic gene ‎improved androgen- phenotype and genotype in rats prostate cancer cells.‎‏ ‏Forty male ‎rats (n=8) weighing approximately 95-100 g were purchased from Pasteur Inistitute of ‎Tehran . Animals were suffered to prostate cancer by injecting LNCaP cell line for 12 ‎weeks. Prostate cancer animals were immediately received endostatin for 28 days. ‎Prostate cancer animals were received stem cell containing the Tsp-1 anti-angiogenesis ‎gene. Histological examinations were measured. The levels of IL8 and VEGF serum ‎were assayed.‎‏ ‏In group receiving cells and drug, amount and height of acinus wall folds ‎decreased compared to the patient group and the shape of the vesicles was clear and the ‎epithelial lining were cohesive and regular. The levels of IL8 and VEGF serum were ‎significantly decreased in combination group than to prostate cancer group (P≤0.01). ‎
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