Study of Biological, compound and Surface Topography if Granule Scaffold Hydroxyapatite/Ostrich Eggshell for Bone Tissue Engineering

Subject Areas : Journal of Animal Biology

Farideh Alahgahi ¹ , Esmail Fattahi ² , Mohsen Saeedi ³ , Mir Mahmoud Mortazavi Roudmiane ⁴

1 - Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 - Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3 - Stem Cell Research Center, Golestan University of Medical sciences, Gorgan, Iran
4 - Transmission Electron Microscope Lab, Biomedical Technology Wing, Sree Chitra Tirunal Institute

Received: 2020-11-05 Accepted : 2021-01-21 Published : 2021-05-22

Keywords: ostrich, Tissue Engineering, egg shell, Hydroxyapatite scaffold, Fibroblast-like cells,

Abstract :

This study aimed to investigate the topography surface and ability of this scaffold in combination with the mesenchymal and fibroblast cells. In this regard, several different ratios of hydroxyapatite / ostrich eggshell scaffold were created to determine the surface properties and responsiveness to the cells with different types of hydroxyapatite/ostrich eggshell (OsE/HA) combinations (ratios 0-1, 1-2, 1-1, 2-1, 1-0). Natural biomaterials including ostrich eggshells which contain the minerals such as calcium carbonate, play a major role in the fabrication of hard tissue scaffolds. Surface characterization was investigated using SEM and recognizing the scaffold properties by XRD, FTIR. Finally, direct contact (toxicity) test with fibroblast-like cells (L929) was performed to study the cytotoxicity response. After the scaffold toxicity test, the material was ready for in vitro acceptance test, which mesenchymal stem cells isolated from the adipose tissue of New Zealand white rabbit (RADMSCs) which kept with this bioscaffold. This study's results showed that this scaffold has the best biocompatibility and the highest surface lacuna for cell adsorption and attachment with hydroxyapatite / ostrich eggshell. Bioscaffold OsE/HA with the ratio of 1-2 has the best cell attachment among other groups. Natural biomaterials with synthetic materials can promote the bone tissue engineering in many ways.

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