Evaluation the Role of GABAA and GABAB Receptors in Anxiety-like Behaviors Caused by Lipopolysaccharides in the Dark and Light Box (LDB) Test in Male Mice

Subject Areas : Journal of Animal Biology

Masoumeh  Alishahi ¹ (Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran)
Maryam  Bananej ² (Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran)
Jalal  Solati ³ (Department of Biology, Islamic Azad University, Karaj Branch, Karaj, Iran)
Ramin  Hajikhani ⁴ (Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran)
Mostafa  Ghaderic ⁵ (Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran)

Received: 2020-05-09 Accepted : 2020-09-04 Published : 2020-11-21

Keywords: GABAA, Lipopolysaccharide, Anxiety-like Behavior, GABAB, Dark and Light Box,

Abstract :

Thegabaergic system is considered as the anti-anxiety system in the brain. The aim of this study was to investigate the role of GABAA and GABAB receptors in anxiety-like behaviors caused by lipopolysaccharides in male mice. In this test,mice were injected with lipopolysaccharide (LPS) (0.2 mg/kg), then injected with either muscimol (0.05, 0.1 or 0.2 µg/mouse), bicuculline (0.25, 0.5 or1 µg/mouse), baclofen (1, 2 or 4 µg/mouse) or CGP (0.2, 0.4 or 0.8 µg/mouse) 2 hours later and received intraventricular Celebrex. Five minutes after intraventricular injection, a dark and light box test was performed. The results of this study showed that musimol significantly reduced anxiety-like behavior in the LDB test (p < 0.05). The results of this study showed that musimol significantly reduced anxiety-like behavior in the LDB test (p < 0.05). However, the administration of musimol and LPS did not significantly change the animal’s anxiety-like behavior (p ≥ 0.05). Different doses of bioculin and LPS significantly increased the anxiety-like behavior in the LDB test (p < 0.05). Baclofen and CGP alone and with LPS did not significantly change the animal’s anxiety-like behavior on the LDB test (p ≥ 0.05). Celebrex injection after LPS relieved the anxiety-like behavior caused by LPS injection. In general, it can be claimed that anxiety-like behaviors in animals receiving LPS may be due to inhibition of GABAA and GABAB receptors and increase in the level of inflammatory factors in brain tissue.

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