Evaluation of the Effects of Methamphetamine Drug on Cell Growth of Stem cells Derived from Uterine Endometrial Tissue of Rats
Subject Areas : Journal of Animal Biology
Zahra Goudarzi
1
(Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran)
Seyyed Ebrahim Hosseini
2
(1- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran)
,Davood Mehrabani
3
(Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran)
Sara Hashemi
4
(Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran)
Keywords: Rat, Stem cell, Endometrium, Methamphetamine, Cell growth,
Abstract :
Methamphetamine is a hallucinogenic substance abused by millions of people all around the world. Cellular toxicity in some cell lines is one of the reported effects of this psychoactive substance. Therefore, this study was conducted to investigate the effect of methamphetamine on the growth of adult mesenchymal stem cells extracted from uterine endometrial tissue in rats. In this experimental study, stem cells were extracted from uterine endometrial tissue in rats, and after cultivation, they were investigated in terms of being mesenchymal using flucytometry method and by CD34, CD90 and CD105 markers. In the third passage of cell culture, the effect of methamphetamine toxicity at a concentration of 0.6 mmol was investigated in a 1-8 day period on the growth of mesenchymal stem cells derived from uterine endometrial tissue by formula PDT= T× . Cells isolated from uterine endometrial tissue adhered completely to the floor of the flask 24 hours after being transferred to the cell culture flask. These cells were positive for non-hematopoietic surface marker expression (CD90, CD105) and negative in terms of hematopoietic marker (CD34) expression, so the mesenchymal identity of these cells was confirmed. The results of the cell count test also showed a significant reduction in the growth of cells treated with 0.6 mmol methamphetamine compared to the control group. The results of this study showed that the cells isolated from the uterine endometrial tissue were of the basic mesenchymal type. The results of cell counting revealed that methamphetamine can inhibit growth by inducing the effects of cell toxicity.
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