Evaluation of GAS8-AS1 Long Non-Coding RNA and GAS8 Target Gene Expression in Patients with Multiple Sclerosis

Subject Areas : Journal of Animal Biology

Mona Patoughi ¹ (Science and Research Branch, Islamic Azad University, Tehran, Iran)
Soudeh Ghafouri-Fard ² (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran)

Received: 2020-04-04 Accepted : 2020-07-23 Published : 2020-09-15

Keywords: Gene expression, Multiple Sclerosis, GAS8, GAS8-AS1,

Abstract :

Expression of the growth arrest specific 8 (GAS8) and its naturally occurring antisense RNA (asRNA) (GAS8-AS1) was assessed in tumoral tissues of different origins. However, their association with immune-related disorders has been poorly understood. In the current study, we evaluated the expression levels of these genes in 50 relapsing remitting multiple sclerosis (RRMS) patients compared with age and sex matched control. Expression assays were performed on peripheral blood samples of study participants following extraction of total RNA and cDNA synthesis using blood. Measurements were then made using the TaqMan (Real Time PCR) method with specific primers and probes sequences. Expression of both genes was significantly higher compared to controls (P=0.001 and p  

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