Morphological Change of Kidney after Injection of the Biological Gold Nanoparticles in Wistar Rats

Subject Areas : Journal of Animal Biology

Behrooz Yahyaei ^{1 *} , Parastoo Pourali ² , Maryam Hassani ³

1 - Department of Medical Sciences, Islamic Azad University, Shahrood Branch, Shahrood, Iran
2 - Department of Medical Sciences, Branch, Islamic Azad University, Shahrood, Iran
3 - Department of Medical Sciences, Islamic Azad University, Shahrood Branch, Shahrood, Iran

Received: 2020-06-03 Accepted : 2020-08-26 Published : 2020-11-21

Keywords: Bacteria, Gold Nanoparticle, kidney, Cell toxicity,

Abstract :

Gold nanoparticles have different uses in medicine. Since the produced chemical nanoparticle is reviewed but the character of the biological one is not checked sufficiently. Therefore, this study on the poisonous effects of the cells in the produced biological nanoparticles is done on the rat’s kidney. After preparing bio-gold nanoparticles from Bucillus subtilis and sterilization bacteria, the nanoparticles were calculated in toxic and non-toxic doses and injected into rats at two doses in 48 hours. Then, after 24 hours, tissue sampling and histological examination were performed. The nonpoisonous nanoparticle injection caused the glomerulus to be smaller & contracted but the other assumes are normal. Nevertheless, the poison injection caused the glomerulus to be fewer in some parts. Dilatation and enlargement was seen in urine space and some glomeruli are smaller and more compressed. The kidneys tubules had no problems but there was a little hyperemia in interstitial space. The usage of biological nanoparticles via the non-biological one had little poisonous effects and the poisonous effects were dosage related.

References:

1. Bashir M Jarrar., Mosaid A. Alferah., 2014. Renal Histological Alterations Induced by 10 nm Gold Nanoparticles Toxicity in Relation with the Time of Exposure. Latin American Journal of Pharmacy, 33(5):725-30.


2. Bruins R., Kapil S., Oehme S., 2000. Microbial resistance to metals in the environment. Ecotoxicology and Environmental Safety, 45(3):198–207.


3. Burdick, G., 1963. Energy Band Structure of Copper. Physical Review, 129: 138.


4. Doudi M., Setorki M., 2014. The effect of gold nanoparticle on renal function in rats. Nanomedicine Journal, 1(3):171-179.


5. Farahnak zarabi M., Saffari Z., Akbarzadeh A., 2014. Synthesis and Applications toward Biology, Catalysis, and medicine Gold Nanoparticles. New Cellular and Molecular Biotechnology Journal, 4(13): 9-20.


6. Freestone I., Meeks N., Sax M., Higgitt C., 2007. The lycurgus cup a Roman nanotechnology. Gold Bulletin, 40: 270-277.


7. Gericke M., Pinches A., 2006. Biological synthesis of metal nanoparticles, Hydrometallurgy. Journal Nanoparticles, 83: 132-140.


8. Harrison R., 2002. Measurment of number massand size disturbtionof particles in the atmosphere. Land, 2567-2580.


9. Kathiresan K., Manivannan S., Nabeel M., Dhivya B., 2009. Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids Surfaces, 71: 133-137.


10. Kumar S., Abyaneh M., Gosavi S., Kulkarni S., Ahmad A., Khan M., 2007. Sulfite reductase-mediated synthesis of gold nanoparticles capped with phytochelatin. Biotechnology and Applied Biochemistry, 47: 191-195.


11. Mohamed Anwar K., Abdelhalim a., Sherif A., Abdelmottaleb Moussa., 2013. The gold nanoparticle size and exposure duration effect on the liver and kidney function of rats: In vivo. Saudi Journal of Biological Sciences, 20(2): 177–181.


12. Rezaei A., Pourali P., Yahyaei B., 2016. Assessment of the cytotoxicity of gold nanoparticles produced by Bacillus cereus on hepatocyte and fibroblast cell lines. Journal of Cellular and Molecular Researches, 29(3): 291-301.


13.  Reznikov A.G., Salivonyk O.A., Sotki, A.G., Shuba Y. M., 2015. Assessment of gold nanoparticle effect on prostate cancer LNCaP cell. Experimenta Oncology, 37(2): 100-104.


14.  Tullo A., 2009. Stretching Tires' Magic Triangle. Chemical and Engineering News. 46:10-14.


15.  Wang Y., He X., Wang K., Zhang X., Tan W., 2009. Barbated Skullcup herb extract-mediated biosynthesis of gold nanoparticles and its primary application in electrochemistry. Biointerfaces, 73(1):75-79.


16.  Yahyaei B., Pourali P., Bakherad S., 2019. Study of the possible effects of the biologically produced gold nanoparticles by Bacillus cereus on the function and structure of the rat`s liver. Journal of Knowledge and Health in Basic Medical Sciences, 13(4): 3-10.


17.  Zhang X., Yan S., Tyagi R., Surampalli R., 2011. Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates. Chemosphere, 82: 489-494.