• Home
  • Evaluation of Anti-Proliferative and Anti- Effects of Kombucha Tea Solvent Fraction on Colorectal Cancer Cell Line (HT-29)

Share To

Article Url


Manuscript ID : ASCIJ-2005-1165 (R1) Visit : 334 Page: 85 - 100

Article Type: Original Research

Evaluation of Anti-Proliferative and Anti- Effects of Kombucha Tea Solvent Fraction on Colorectal Cancer Cell Line (HT-29)

Subject Areas : Journal of Animal Biology

Elham Ghodousi Dehnavi 1 , Mohammad Arjmand 2 , Reza Haji Hosseini 3 , Zahra Zamani 4 , Sima Nasri 5

1 - Department of Biology, Payame Noor University (PNU), Tehran-East, Tehran, Iran
2 - Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
3 - Department of Biology, Payame Noor University (PNU), Tehran-East, Tehran, Iran
4 - Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
5 - Department of Biology, Payame Noor University (PNU), Tehran-East, Tehran, Iran

Received: 2020-05-25 Accepted : 2020-09-10 Published : 2020-11-21

Keywords: Apoptosis, anti-proliferative, Kambucha Tea, DNA Fragmentation, clonogenic,

Abstract :

Fermented beverage, traditionally the result of the fermentation of sweet tea by Kombucha fungus, has beneficial effects in the treatment of many diseases, especially cancer. The present study is aimed at assessing the effect of different fractions of Kombucha tea on proliferation and apoptosis on colon cancer cell line HT-29. In the present experimental study, the survival rate and cell proliferation of Kumbucha tea solvent fractions including chloroform, ethyl acetate, butanol, hexane, and the final aqueous phase at concentrations of 0-900 μg/ ml were investigated by MTT and clonogenic assay. The rate of apoptosis induction was assessed by DNA fragmentation assay and flowcytometry. The aqueous and butanol fractions had no cytotoxic effects. After 24 hours, the IC50 for ethyl acetate, chloroform, and hexane was determined 213/49±1/63, 296/70±2/11 and 563/2±83/29 μg/ml, respectively. The results revealed that inhibition of cell growth was dose-dependent. The concentration of IC50 in these fractions caused the DNA to fragment. Apoptosis analysis by flowcytometry with these concentrations showed that the ethyl acetate component caused apoptosis while the death caused by IC50 concentrations of chloroform and hexane fractions was more than necrosis. The dose-dependent ethyl acetate fraction of Kambucha tea induced cell death through the apoptotic pathway and it was observed to be responsible for the anti-apoptotic and anti-proliferative properties. It seems to be a good candidate to prevent the proliferation of colorectal cancer cells.  

References:


  1. Aggarwal B.B., Harikumar K.B. 2009. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. The International Journal of Biochemistry and Cell Biology, 41(1): 40-59.
    2.
  2. Alshatwi A.A. 2010. Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis. Journal of Experimental and Clinical Cancer Research, 29(1): 1-9.
    3.
  3. Anboutaleb A., Soheir S., Abdel Salam N.M. 2017. Antimicrobial and antiproliferative, pro-apopiotic actions of kumbucha fermented solution against colon and hepato cancer line. World Journal of Pharmaceutical and Life Sciences, 32(3): 120-132
    4.
  4. Antoni S., Ferlay J., Soerjomataram I., Znaor A., Jemal A., Bray F. 2017. Bladder cancer incidence and mortality: a global overview and recent trends. European Urology, 71(1): 96-108.
    5.
  5. Arnold M., Sierra M.S., Laversanne M., Soerjomataram I., Jemal A., Bray F. 2017. Global patterns and trends in colorectal cancer incidence and mortality. Gut, 66(4): 683-691.
    6.
  6. Benarba B., Pandiella A. 2018. Colorectal cancer and medicinal plants: Principle findings from recent studies. Biomedicine and Pharmacotherapy, 107: 408-423.
    7.
  7. Bhardwaj M., Paul S., Jakhar R., Kang S.C. 2015. Potential role of vitexin in alleviating heat stress-induced cytotoxicity: Regulatory effect of Hsp90 on ER stress-mediated autophagy. Life sciences, 142: 36-48.
    8.
  8. Cetojevic-Simin D.D., Bogdanovic G.M., Cvetkovic D.D., Velicanski A.S. 2008. Antiproliferative and antimicrobial activity of traditional Kombucha and Satureja montana L. Kombucha. J. BUON, 13(3): 395-401.
    9.
  9. Četojević-Simin D.D., Velićanski A.S., Cvetković D.D., Markov S.L., Mrđanović J.Ž., Bogdanović V.V., Šolajić S.V. 2012. Bioactivity of lemon balm kombucha. Food and Bioprocess Technology, 5(5): 1756-1765.
    10.
  10. Chakravorty S., Bhattacharya S., Chatzinotas A., Chakraborty W., Bhattacharya, D., Gachhui R. 2016. Kombucha tea fermentation: Microbial and biochemical dynamics. International Journal of Food Microbiology, 220: 63-72.
    11.
  11. Choi H.J., Eun J. S., Kim B.G., Kim S. Y., Jeon H., Soh Y. 2006. Vitexin, an HIF-1α Inhibitor, Has Anti-Metastatic Potential in PC12 Cells. Molecules and Cells, 22(3):
    12.
  12. De Roos J., De Vuyst L. 2018. Acetic acid bacteria in fermented foods and beverages. Current opinion in biotechnology, 49: 115-119.
    13.
  13. Dickson M.A., Schwartz, G.K. 2009. Development of cell-cycle inhibitors for cancer therapy. Current Oncology, 16(2): 36.
    14.
  14. Dufresne C., Farnworth E. 2000. Tea, Kombucha, and health: a review. Food research international, 33(6): 409-421.
    15.
  15. Elmore S. 2007. Apoptosis: a review of programmed cell death. Toxicologic Pathology, 35(4): 495-516.
    16.
  16. Engelhardt U.H., Finger A., Kuhr S. 1993. Determination of flavone C-glycosides in tea. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 197(3): 239-244.
    17.
  17. Focaccetti C., Bruno A., Magnani E., Bartolini D., Principi E., Dallaglio K., Albini A. 2015. Effects of 5-fluorouracil on morphology, cell cycle, proliferation, apoptosis, autophagy and ROS production in endothelial cells and cardiomyocytes. PloS one, 10(2): e0115686.
    18.
  18. Frankfurt O.S., Krishan A. 2003. Apoptosis-based drug screening and detection of selective toxicity to cancer cells. Anti-cancer drugs, 14(7): 555-561.
    19.
  19. Fulda S. 2010. Modulation of apoptosis by natural products for cancer therapy. Planta medica, 76(11): 1075-1079.
    20.
  20. Li G., Lou H.X. 2018. Strategies to diversify natural products for drug discovery. Medicinal Research Reviews, 38(4): 1255-1294.
    21.
  21. Gandomani H.S., Yousefi S.M., Aghajani M., Mohammadian-Hafshejani A., Tarazoj A.A., Pouyesh V., Salehiniya H. 2017. Colorectal cancer in the world: incidence, mortality and risk factors. Biomedical Research and Therapy, 4(10): 1656-1675.
    22.
  22. Honardoost M., Soleimanjahi H., Rajaei F. 2013. Apoptosis: programmed cell death. Journal of Qazvin University of Medical Sciences, 17(3): 48-57.
    23.
  23. Huang X.M., Yang Z.J., Xie Q., Zhang Z.K., Zhang H., Ma J.Y. 2019. Natural products for treating colorectal cancer: A mechanistic review. Biomedicine and Pharmacotherapy, 117:109-142.
    24.
  24. Huh S.W., Bae S.M., Kim Y.W., Lee J.M., Namkoong S.E., Lee I.P., Ahn W.S. 2004. Anticancer effects of (−)-epigallocatechin-3-gallate on ovarian carcinoma cell lines. Gynecologic oncology, 94(3): 760-768.
    25.
  25. Jayabalan R., Malbaša R.V., Lončar E.S., Vitas J.S., Sathishkumar M. 2014. A review on kombucha tea-microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4): 538-550.
    26.
  26. Jayabalan R., Chen P. N., Hsieh Y.S., Prabhakaran K., Pitchai P., Marimuthu S., Yun S.E. 2011. Effect of solvent fractions of kombucha tea on viability and invasiveness of cancer cells—characterization of dimethyl 2-(2-hydroxy-2-methoxypropylidine) malonate and vitexin. Indian Journal of Biotechnology, 10(1): 75-82.
    27.
  27. Kemp K., Griffiths J., Campbell S., Lovell K. 2013. An exploration of the follow-up up needs of patients with inflammatory bowel disease. Journal of Crohn's and Colitis, 7(9): e386-e395.
    28.
  28. Kim C.I., Shin S.S., Park S.S. 2016. Growth inhibition and induction of apoptosis in human bladder cancer cells induced by fermented citrus Kombucha. Journal of the Korean Society of Food Science and Nutrition, 45(10): 1422-1429.
    29.
  29. Koosha S., Alshawsh M.A., Looi C. Y., Seyedan A., Mohamed Z. 2016. An association map on the effect of flavonoids on the signaling pathways in colorectal cancer. International Journal of Medical Sciences, 13(5): 374.
    30.
  30. Koosha S., Mohamed Z., Sinniah A., Alshawsh M.A. 2019. Investigation into the Molecular Mechanisms underlying the Anti-proliferative and Anti-tumorigenesis activities of Diosmetin against HCT-116 Human Colorectal Cancer. Scientific Reports, 9(1): 1-17.
    31.
  31. Korir M.W., Wachira F.N., Wanyoko J.K., Ngure R.M., Khalid R. 2014. The fortification of tea with sweeteners and milk and its effect on in vitro antioxidant potential of tea product and glutathione levels in an animal model. Food Chemistry, 145: 145-153.
    32.
  32. Leal J.M., Suárez L.V., Jayabalan R., Oros J.H., Escalante-Aburto A. 2018. A review on health benefits of kombucha nutritional compounds and metabolites. CyTA-Journal of Food, 16(1): 390-399.
    33.
  33. Liang Y.C., Chen Y.C., Lin Y.L., Lin-Shiau S.Y., Ho C.T., Lin J.K. 1999. Suppression of extracellular signals and cell proliferation by the black tea polyphenol, theaflavin-3, 3′-digallate. Carcinogenesis, 20(4): 733-736.
    34.
  34. Mann J. 2002. Natural Products in Cancer Chemotherapy: past, present and future–Nature Reviews. Cancer, 2: 145.
    35.
  35. Meadows G.G. 2012. Diet, nutrients, phytochemicals, and cancer metastasis suppressor genes. Cancer and Metastasis Reviews, 31(3-4): 441-454.
    36.
  36. Newman D.J., Cragg G.M. 2012. Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of natural products, 75(3): 311-335.
    37.
  37. Palozza P., Serini S., Maggiano N., Angelini M., Boninsegna A., Di Nicuolo F., Calviello G. 2002. Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by β-carotene through down-regulation of cyclin A and Bcl-2 family proteins. Carcinogenesis, 23(1): 11-18.
    38.
  38. Paterson I., Anderson E.A. 2005. The renaissance of natural products as drug candidates. Science, 310(5747): 451-453.
    39.
  39. Sreeramulu G., Zhu Y., Knol W. 2000. Kombucha fermentation and its antimicrobial activity. Journal of Agricultural and Food Chemistry, 48(6): 2589-2594.
    40.
  40. Srihari T., Arunkumar R., Arunakaran J., Satyanarayana U. 2013. Downregulation of signalling molecules involved in angiogenesis of prostate cancer cell line (PC-3) by kombucha (lyophilized). Biomedicine and Preventive Nutrition, 3(1): 53-58.
    41.
  41. Tan W., Lu J., Huang M., Li Y., Chen M., Wu G., Guo J. 2011. Anti-cancer natural products isolated from chinese medicinal herbs. Chinese Medicine, 6(1):27.
    42.
  42. Tan Z., Zhang Y., Deng J., Zeng G., Zhang Y. 2012. Purified vitexin compound 1 suppresses tumor growth and induces cell apoptosis in a mouse model of human choriocarcinoma. International Journal of Gynecologic Cancer, 22(3): 360-366.
    43.
  43. Tournigand C., André T., Bonnetain F., Chibaudel B., Lledo G., Hickish T., De Gramont A. 2012. Adjuvant therapy with fluorouracil and oxaliplatin in stage II and elderly patients (between ages 70 and 75 years) with colon cancer: subgroup analyses of the Multicenter International Study of Oxaliplatin, Fluorouracil, and Leucovorin in the Adjuvant Treatment of Colon Cancer trial. Journal of Clinical Oncology, 30(27: 3353-3360.
    44.
  44. Troiani T., Martinelli E., Napolitano S., Vitagliano D., Ciuffreda L.P., Costantino S., De Palma R. 2013. Increased TGF-α as a mechanism of acquired resistance to the anti-EGFR inhibitor cetuximab through EGFR–MET interaction and activation of MET signaling in colon cancer cells. Clinical Cancer Research, 19(24): 6751-6765.
    45.
  45. Vermeulen K., Van Bockstaele D.R., Berneman Z.N. 2005. Apoptosis: mechanisms and relevance in cancer. Annals of hematology, 84(10): 627-639.
    46.
  46. Vīna I., Semjonovs P., Linde R., Deniņa I. 2014. Current evidence on physiological activity and expected health effects of kombucha fermented beverage. Journal of Medicinal Food, 17(2): 179-188.
    47.
  47. Watson, A.J. M. 2004. Apoptosis and colorectal cancer. Gut, 53(11): 1701-1709.
    48.
  48. Yang G.Y., Liao J., Li C., Chung J., Yurkow E.J., Ho C.T., Yang C.S. 2000. Effect of black and green tea polyphenols on c-jun phosphorylation and H2O2 production in transformed and non-transformed human bronchial cell lines: possible mechanisms of cell growth inhibition and apoptosis induction. Carcinogenesis, 21(11): 2035-2039.
    49.
  49. Yang Z.W., Ji B.P., Zhou F., Li B., Luo Y., Yang L., Li T. 2009. Hypocholesterolaemic and antioxidant effects of kombucha tea in high‐cholesterol fed mice. Journal of the Science of Food and Agriculture, 89(1): 150-156.
    50.
_||_

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]