The Effect of the Iron Magnetic Nanoparticles on the Expression of P53 Protein in Brain Tissue of the Balb/C Mice by Immunohistochemically Method

Subject Areas : Journal of Animal Biology

L. Farhadi ¹ , V. Hojati ² , A. Shiravi ³

1 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
3 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

Received: 2017-01-08 Accepted : 2017-05-21 Published : 2017-01-20

Keywords: Brain, Protein53, Magnetic iron nanoparticles, Imonohistochemistry,

Abstract :

Due to physico-chemical properties of nanoparticles as drug carriers are widely used in the treatment of cancerous cells in living environments. The nanoparticles can be used for targeted drug delivery and effective to the site of infection. Iron magnetic nanoparticles have biologically active properties and can enter into the reticuloendothelial system by phagocytosis or endocytosis, or can be trapped by phagocytic cells such as monocytes, macrophages and oligodendrocytes. The nanoparticles can hurt cells by increasing oxidative stress. P53 is a tumor suppressor protein and it is disabled or impaired function in most human cancers. The aim of this study was to evaluate the effect of iron oxide nanoparticles on the expression of P53 protein in the brain tissue of mice Balb/C. For this purpose, 15 male mice were divided into three groups (five in each group). The treatment groups 1 and 2 during 8 days, received doses of 100 and 300 ppm magnetic nanoparticles of iron daily by intraperitoneal injection. At the end of eight days, the animals dissected and their brains were removed and were undergone immunostaining with Avedin. The results showed that iron oxide nanoparticles penetrate into brain tissue and causing inflammatory response and cause increasing in P53 protein. It seems that high concentrations of iron oxide nanoparticles have a significant effect on the expression of P53 protein.