Effect of Alkaline Phosphatase on the Enzymes and Proteins of Liver Tissue of Rat by Enzyme Inhibition
Subject Areas : Journal of Animal Biologyالهه سامانی جهرمی 1 , سمانه ذوالقدری جهرمی 2
1 - باشگاه پژوهشگران جوان و نخبگان، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران
2 - گروه زیست شناسی، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران
Keywords: liver, alkaline phosphatase, Enzyme inhibition, L- homo arginin,
Abstract :
Liver is the largest gland in the body; which have different secretions. Alkaline phosphatase enzyme is one of them. The aim of this study is the investigation of alkaline phosphatase effect on the enzymes and proteins of liver in the male rats on the basis of enzyme inhibition by L-Homo argenine. In this experimental study, 35 adult male rats were studied. They were randomly divided into 5 groups with seven rats in each group, including: control, sham and testing 1, 2 and 3 respectively. Control group were nourished by food and water; Sham group were injected just solvent. Group 1: 0.2 mg/ml alkaline phosphatase enzyme was injected. In Group 2: 2 mg/ml L-homo arginine inhibitors were injected. Group 3: First 2 mg/ml L-homo arginine was injected, then after 2 hours, 0.2 mg/ml alkaline phosphatase injection was done. All injections continued for 5 days. At the end of duration, a sample was prepared for evaluating ALP. Data was collected by using SPSS software and by one-way variance analytical tests (ANOVA) in significance P