The Study of Molecular and Population Diversity of Rutilus frisiikutum in Anzali Lagoon and Shirud River by Using of Molecular Genetic Method

Subject Areas : Journal of Animal Biology

فریدون چکمه دوز قاسمی ¹ , شهرام بهمنش ² , مهتاب یارمحمدی ³ , محمد حسن زاده صابر ⁴

1 - پژوهشکده آبزی پروی آبهای داخلی، بندر انزلی، ایران
2 - پژوهشکده آبزی پروی آبهای داخلی، بندر انزلی، ایران
3 - موسسه تحقیقات بین المللی ماهیان خاویاری دریای خزر، رشت، ایران
4 - موسسه تحقیقات بین المللی ماهیان خاویاری دریای خزر، رشت، ایران

Received: 2014-12-06 Accepted : 2017-05-21 Published : 2014-10-23

Keywords: Anzali lagoon, Caspian sea kutum, Population genetic structure, Microsatellite markers,

Abstract :

Anzali Lagoon and Shirud River are two important spawning areas in south Caspian Sea for kutum. Due to the economically valuable of this fish, molecular and population genetic structure of this fish was investigated using microsatellite markers.For this purpose, 100 samples from two regions were collected and DNA was extracted. Ten pairs microsatellite primer was used for PCR which all made polymorphic patterns. Data resulted: 191 alleles were observed. The maximum numbers of alleles (18) were found in two loci (Ca1 and Ca3) and the minimum number of alleles (2) was found in MFW1 locus. The differences between samples of two regions were not statistically significant (P>0.05) neither for average number of alleles per locus nor for observed heterozygosity. The calculated Fst was 0.056 and significant (P