Influence of intracerebral administration of muscarinic agents in dorsal hippocampus (CA1) on cannabinoid state-dependent memory in the step-down passive avoidance test

Subject Areas : Journal of Animal Biology

مرتضی پیری ¹ , محمدرضا زرین دست ² , مریم السادات شاهین ³

1 - گروه زیست شناسی ، دانشگاه آزاد اسلامی واحد اردبیل
2 - گروه فارماکولوژی، دانشکده پزشکی و مرکز ملی مطالعات اعتیاد، دانشگاه علوم پزشکی تهران
3 - گروه زیست شناسی ، دانشگاه آزاد اسلامی واحد شهر ری و عضو باشگاه پژوهشگران جوان

Received: 2014-06-23 Accepted : 2017-05-21 Published : 2009-05-22

Keywords: Mouse, WIN55, 212-2, Physostigmine, Scopolamine, State-dependent memory, Step-down passive avoidance task,

Abstract :

In the present study, the effects of muscarinic agents on WIN55, 212-2 induced state-dependent memory of passive avoidance task were examined in mice. One-trial step-down paradigm was used for the assessment of memory retention in adult male NMRI mice. Post-training intra-CA1 administration of cannabinoid receptor agonist, WIN55, 212-2 (0.5 and 1 andmicro;g/mouse) decreased the memory retrieval. Amnesia induced by post-training WIN55, 212-2 (1andmicro;g/mouse) was significantly reversed by pre-test administration of WIN55,212-2 (0.5, 1andmu;g/mouse, intra-CA1), and induced WIN55, 212-2-state-dependent memory. Administration of scopolamine (4 andmicro;g/mouse) but not physostigmine (0.25, 1 and 4 andmicro;g/mouse) intra-CA1, 5 min before test by itself decreased the memory retrieval. On the other hands, the animals in which retrieval was impaired, due to WIN55, 212-2 (1andmicro;g/mouse) post-training administration, pre-test administration of physostigmine (1 and 4 andmicro;g/mouse) and scopolamine ( 4 andmicro;g/mouse) intra-CA1 24 hr after train in dayandrsquo;s test restored, memory retrieval. Furthermore animals under influence of post-training administration of WIN55, 212-2 (1andmicro;g/mouse), pre-test co-administration of non-effective doses of WIN55, 212-2 (0.25 andmicro;g/mouse) and scopolamine (1 andmicro;g/mouse), increased the restoration of memory by the WIN55, 212-2. These findings may implicate the involvement of muscarinic receptor in state-dependent memory induced by intra-CA1 administration of the WIN55, 212-2.