Interaction of Antinociceptive Intraperitoneal Injection of Extract of Foeniculum vulgare Fruit and Morphine in Mice

Subject Areas : Journal of Animal Biology

غلامحسن واعظی ¹ , محدثه عموزاد خلیلی ² , کیوان کرامتی ³

1 - دانشیار گروه زیست شناسی، دانشگاه آزاد اسلامی واحد سمنان
2 - دانشجوی کارشناسی ارشد فیزیولوژی دانشگاه آزاد اسلامی واحد دامغان
3 - - استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد دامغان

Received: 2014-06-23 Accepted : 2017-05-21 Published : 2010-01-21

Keywords: Foeniculum vulgare, Morphine, Antinociceptive, Writhing test,

Abstract :

Morphine for years as an analgesic drug is used, but given that the repeated administration of morphine is associated with dependence and tolerance, recent studies try is to administration of aqueous extract of fennel, along with its analgesic effect of morphine and the strengthening of reduce its consumption.This experimental study performed on 80 male mice (28andplusmn;3g). Animals were grouped randomly in to control, positive control and receivers of Foeniculum vulgareextract. Control group and positive control group received normal saline 0.9% and indomethacin (5mg/kg, i.p.), respectively. Treatments groups were injected with doses, (100 mg/kg, i.p.), (200 mg/kg, i.p.) and (400 mg/kg, i.p.) of aqeous extract of Foeniculum vulgare. Animals were injected with acetic acid 0.6% (10ml/kg) for iducing of visceral pain, 30 minutes after of each intraperitoneal administration. Antinociceptive effect was recorded by counting the number of writhes immediately after injection of acetic acid during 30 minutes. In following treatments interaction of morphine in the antinociceptive effects of Foeniculum vulgare extract were studied with treatment ofmorphine (2mg/kg, i.p.) 15 minute after the administration of the extract in writhing test.this study showed that aqueous extract of F. vulgareat 100, 200 and 400 mg/kg and indomethacin (5mg/kg) induced a significant reduction in pain response compared to the control group (pandlt;0.001). morphine (2mg/kg, i.p.) also coused antinociception and the concurrent use of F. vulgareand morphine produced more remarkable antinociception compared to their individual usages (pandlt;0.001).Foeniculum vulgare andmorphine also showed synergistic drug interaction for antinociception in the writhing test.