The Influence of Dietary Lysine Levels on Small Intestine Morphometrics in Starter and Grower Period of Broiler Chickens

Subject Areas : Journal of Animal Biology

غلامحسن واعظی ¹ , مسعود تشفام ² , شهاب بهادران ³ , حیدر فرازیان ⁴

1 - دانشگاه آزاد اسلامی، واحد سمنان، گروه پیراپزشکی، سمنان، ایران
2 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه فیزیولوژی، فارماکولوژی و سم شناسی دانشکده دامپزشکی، تهران، ایران
3 - دانشگاه شهرکرد، دانشکده دامپزشکی، گروه علوم درمانگاهی، شهرکرد، ایران
4 - دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران

Received: 2014-06-22 Accepted : 2017-05-21 Published : 2011-07-23

Keywords: Lysine, small intestine Morphometrics, villi, broilers chickens,

Abstract :

Theandshy; purpose of this study to demonstrate the effects of lysine supplement on Small Intestine Morphometrics of broilers chickens. 180 day old male Ross breed broiler were purchased and divided into 3 groups. In starter period Control group received diet containing 1.2% lysine, Treatment 1 and Treatment 2 group received diet containing 1.3% and 1.4% Lysine, respectively. In Grower period Control group received diet containing 1.1% lysine, Treatment 1 and Treatment 2 group received diet containing 1.2% and 1.3% Lysine, respectively. Intestine villous Morphometrics examination was done by analyzing of villiand#39;s height, width and depth of crypts. PAS solution was used in order to staining samples. Data analyzed by SPSS 17software through one-way variance analysis and Tukey Test. The finding shown that as diet lysine increased, in trearment2 the villous height and crypt depth had a significant increase compared to the control Group. The growth of intestine villous width which depends on lysine density had significant increase (Pandlt;0.05) compared to control group. Lysine had no significant effect villous height to crypt depth ratio and villous height to villous width ratio in the jejunum.