Cytotoxicity Effects of Bishops Flower (Ammi majus) Extract on the Cancer Cell Lines Hela and MCF-7
Subject Areas : Journal of Animal Biology
فرخنده
نعمتی
1
(دانشگاه آزاد اسلامی، واحد قائمشهر،گروه زیست شناسی، مرکز تحقیقات سلولی ـ مولکولی، قائمشهر، ایران)
بهمن
اسلامی جدیدی
2
(انشگاه آزاد اسلامی، واحد قائمشهر،گروه زیست شناسی، مرکز تحقیقات سلولی ـ مولکولی، قائمشهر، ایران)
مهدیه
طالبی دارابی
3
(دانشگاه آزاد اسلامی، واحد قائمشهر،گروه زیست شناسی، مرکز تحقیقات سلولی ـ مولکولی، قائمشهر، ایران)
Keywords: Cytotoxicity, Hela cell line, Etanolic extraction, Ammi majus, MCF7cell line,
Abstract :
Todaymany of the treatment methods are used to treat cancer. Unfortunately, in most cases very poor response is often associated with undesirable side effects. AlphaLack ofresponse to treatment and the rapid growth of the disease, has been made researchers to achieve more effective drugs with fewer side effects. In this study the effect of ethanolic extraction of theBishopand#39;s Flower, Ammi majus on the cancer cell lines Hela and MCF-7 were examined. Cells were cultured in cell culture flasks then transferred to 96 well plates with 10,000 cells. Cells were exposed to different concentrations of Ammi majus. The cytotoxicity of Ammi majus was assessed using the MTT assay after 72 hours. The results of this study showed that ethanolic extract of Ammi majus on Hela cells was significantly reduced the cells grow compared with controls after 72 h. The calculated IC50, was 1/922 mg / ml. The cytotoxicity effect this extract on MCF7 showed that Ammi majus was significantly reduced the MCF7 cells grow compared with controls after 72 h. The calculated IC50, was 10 mg / ml. The effect of this extracts on normal cells (lymphocytes and monocytes in blood) is granted, the results show that the concentration of 10 mg / ml extracts had no effect on normal cells. The results suggest that ethanolic extract of the plant Ammi majus inhibits growth of cancer cells MCF7 and Hela.