Effect of Aloe vera Extract on Spermatogenesis and Reproductive Hormones in Mice

Subject Areas : Journal of Animal Biology

مهرداد مدرسی ¹ , حمیدرضا خدائی ² , علیرضا خدادادی ³

1 - دانشگاه آزاد اسلامی، واحد خوراسگان (اصفهان)، گروه علوم دامی، اصفهان، ایران
2 - دانشگاه آزاد اسلامی، واحد گلپایگان، گروه علوم دامی، گلپایگان، ایران
3 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه علوم دامی، تهران، ایران

Received: 2014-06-21 Accepted : 2017-05-21 Published : 2013-10-23

Keywords: Testosterone, Aloe veraextract, stem cells, primary spermatocyte, testicularweights,

Abstract :

Aloe with the scientific name Aloe vera is a perennial plant that has useful effects in herbal therapy and herbal medicine. In this research the effects of hydroalcoholic extract of Aloe vera gel on the testis and spermatogenesis in mice Balb/C mice were examined. Samples randomly divided into five groups (three treatment groups, placebo and control) and all samples were kept in the same conditions. Aloe vera gel extract in different doses (50, 100 and 200 mg/kg/2day) produced by intraperitoneal (IP) for 20 days was administered to the treatment group. Placebo group was used as the injection of saline. The main parameters that were examined changes in levels of testosterone, LH and FSH levels and changes in stem cell number, number primary spermatocytes, and testis weight compared with the control group. All of hormonal experiments using Radioimmunoassay (RIA) were performed.The results showed that Aloe vera extract caused a significant increase in the number of stem cells and primary spermatocytes in the experimental group compared with the control group. Mean of testicular weights and testosterone levels in group 3 (200 mg/kg) increased significantly, while LH and FSH levels did not show significant changes. The findings indicate Aloe vera extract in a dose-dependent effect on testosterone levels and histological characteristics of male mice.