Effect of Tamoxifen on Oogenesis of the Female Wistar Rats
Subject Areas : Journal of Animal Biologyزهرا کشتمند 1 , شهربانو عریان 2 , کاظم پریور 3
1 - دانشجوی دکترافیزیولوژی جانوری، دانشگاه آزاداسلامی واحد علوم و تحقیقات تهران
2 - دکترای فیزیولوژی جانوری، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران
3 - دکترای تکوین جانوری، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران
Keywords: Rat, Oogenesis, Tamoxifen,
Abstract :
Tamoxifen is a nonsteroidalantiestrogen which is prescribed for treatment of breast cancer. Some negative effects of this drug on the reproductive system have been observed.The major aim of this study is to investigate the effect of Tamoxifen on the oogenesis in female Wistar rats. Three group of rats (250 gr range ) gavaged with Tamoxifen doses of 200, 400 and 600 andmicro;g/kg B.W(exp.1, exp.2, exp.3 ) dissolved in solvent [ethanol 60 % and physiological solution] for 30 days. The sham group was gavaged with solvent and control did not receive solvent or drug. On days 1and 30 after the end of treatment, sections of ovary stained with Handamp;E method and were observed histologically. Results of the observation were analyzed by the one-way ANOVA, Tukey test and using SPSS software determined andplusmn;SEthe means Result were termed significant if Pandle;0.05. In the first sampling number of primordial follicle, secondary follicle ,graafian follicle and corpus luteum were decreased significantly in experimental groups which received Tamoxifen compared with control group [pandlt;0.001]. In the second sampling, number of primordial follicle secondary follicle, graafian follicle and corpus luteum increased compared with first sampling. The numbers of offsprings in experimental groups were less than control litters.These findings suggest thatTamoxifen decreases the fertilization ability and has negative effects on the oogenesis in female Wistar rats. Some of negative effects of Tamoxifen on reproduction ability, is abolished with time elapsed.