Achillea Santolina Hydroalcoholic Extract Induces Cell Death in Breast Cancer MCF-7 Cells Cultured in Fibrin Gel

Subject Areas : Journal of Animal Biology

Elham Hoveizi ¹ (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran)
Peyman Abdolali nejad ² (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran)

Received: 2022-07-26 Accepted : 2022-09-24 Published : 2023-05-22

Keywords: breast cancer, Achillea Santolina, cell viability, Hydrogel Scaffold,

Abstract :

 It is a continuing effort to develop new anticancer compounds. Some studies have reported that some compounds of Achillea Santolina have inhibitory effects on several cancer cell lines. The present study aims to evaluate the cytotoxic effect of the hydroalcoholic extract of Achillea Santolina on MCF-7 human breast cancer cells in fibrin hydrogel. In this experimental study, to prepare the fibrin hydrogel scaffold, an M199 medium containing 10% FBS, 1% penicillin/streptomycin, fibrinogen powder with a concentration of 3 mg/ml, and thrombin with a concentration of 120 u/ml was used. Cultured MCF-7 cells in fibrin gel were treated with hydroalcoholic extract of Achillea Santolina in 25, 50, 100, 250, 500, and 1000 mg/ml concentrations for 24h. Morphology and cellular viability and proliferation were evaluated by DAPI staining and MTT assay at certain days after treatment. Also, the structure of the scaffold and the condition of the cells in the gel were investigated by photographing with a scanning electron microscope.The results of this study showed the cytotoxic effects of Achillea Santolina extract in a dose- and time-dependent manner. The concentration of 500 mg/ml of the extract of A. Santolina was determined as an IC50 concentration. Also, cell viability in the control group and treated group with extract at IC50 concentrations showed significant (P<0.05) differences on days 1, 3, and 5. The hydroalcoholic extract of Achillea Santolina can inhibit MCF-7 cell proliferation and induce cell death in a dose and time-dependent manner.

References:

1. Ahn K.I., Choi E.O., Kwon D.H., HwangBo H., Kim M.Y., Kim H.J., Ji S.Y., Hong S.H., Jeong J.W., Park C., Kim N.D., Kim W.J., Choi Y.H., 2017. Induction of apoptosis by ethanol extract of Citrus unshiu Markovich peel in human bladder cancer T24 cells through ROS-mediated inactivation of the PI3K/Akt pathway. BioScience Trends, 11(5):565-573.


2. Albayrak S., 2013. The Volatile Compounds and Bioactivity of Achillea sieheana Stapf. (Asteraceae). Iranian Journal of Pharmaceutical Research, 12(1): 37-45.


3. Bayat N., Ebrahimi-Barough S., Ardakan M.M., Ai A., Kamyab A., Babaloo H., Ai J., 2016. Differentiation of Human Endometrial Stem Cells into Schwann Cells in Fibrin Hydrogel as 3D Culture. Molecular Neurobiology, 53(10):7170-7176.


4. Bayat N., Izadpanah R., Ebrahimi-Barough S., Norouzi Javidan A., Ai A., Mokhtari Ardakan M.M., Saberi H., Ai J., 2018. The Anti-Angiogenic Effect of Atorvastatin in Glioblastoma Spheroids Tumor Cultured in Fibrin Gel: in 3D in Vitro Model. Asian Pacific Journal of Cancer Prevention, 19(9):2553-2560.


5. Chen Y., Lin J.S., 2017. The application of aptamer in apoptosis. Biochimie, 132:1-8.


6. Ebrahimi M., Farajpour M., Rahimmalek M., 2012. Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers. The Genetics and Molecular Research, 11(3):2855-2861.


7. Farmani F., Moein M., Amanzadeh A., Kandelous H.M., Ehsanpour Z., Salimi M., 2016. Antiproliferative Evaluation and Apoptosis Induction in MCF- 7 Cells by Ziziphus spina christi Leaf Extracts. Asian Pacific Journal of Cancer Prevention, 17(1):315-321.


8. Galavi H.R., Saravani R., Shahraki A., Ashtiani M., 2016. Anti-proliferative and apoptosis inducing potential of hydroalcoholic Achillea wilhelmsii C. Koch extract on human breast adenocarcinoma cell lines MCF-7 and MDA-Mb-468. Pakistan Journal of Pharmaceutical Sciences, 29(6 Suppl):2397-2403.


9. Harbeck N., 2018. Advances in targeting HER2-positive breast cancer. Current Opinion in Obstetrics and Gynecology, 30(1):55-59.


10. Hassett M.J., Li H., Burstein H.J., Punglia R.S., 2020. Neoadjuvant treatment strategies for HER2-positive breast cancer: cost-effectiveness and quality of life outcomes. Breast Cancer Research and Treatment, 181:43-51.


11. Hoveizi E., Ebrahimi-Barough S., Tavakol S., Nabiuni M., 2015. In vitro comparative survey of cell adhesion and proliferation of human induced pluripotent stem cells on surfaces of polymeric electrospun nanofibrous and solution-cast film scaffolds. Journal of biomedical materials research Part A, 103(9): 2952-2958.


12. Jamalzadeh L., Ghafoori H., Aghamaali M., Sariri R., 2017. Induction of Apoptosis in Human Breast Cancer MCF-7 Cells by a Semi-Synthetic Derivative of Artemisinin: A Caspase-Related Mechanism. Iranian Journal of Biotechnology, 15(3):157-165.


13. Khafagy S.M., el Fatatry L.A., Amer M. S., 1965. Preliminary characterization of santolin, bitter principle of Achillea santolina L. growing in Egypt. Acta Pharmaceutica Sinica B, 2(6):403-410.


14. Kim H., Moon J.Y., Mosaddik A., Cho S.K., 2010. Induction of apoptosis in human cervical carcinoma HeLa cells by polymethoxylated flavone-rich Citrus grandis Osbeck (Dangyuja) leaf extract. Food and Chemical Toxicology, 48(8-9): 2435-2442.


15. Kim M.Y., Bo H.H., Choi E.O., Kwon D.H., Kim H.J., Ahn K.I., Ji S.Y., Jeong J.W., Park S.H., Hong S.H., Kim G.Y., Park C., Kim H.S., Moon S.K., Yun S.J., Kim W.J., Choi Y.H., 2018. Induction of Apoptosis by Citrus unshiu Peel in Human Breast Cancer MCF-7 Cells: Involvement of ROS-Dependent Activation of AMPK. Biological and Pharmaceutical Bulletin, 41(5):713-721.


16. Liu C., Wu F., Liu Y., Meng C., 2015. Catalpol suppresses proliferation and facilitates apoptosis of MCF-7 breast cancer cells through upregulating microRNA-146a and downregulating matrix metalloproteinase-16 expression. Molecular Medicine Reports, 12(5):7609-7614.


17. Mirzapur P., Khazaei M.R., Moradi M.T., Khazaei M., 2018. Apoptosis induction in human breast cancer cell lines by synergic effect of raloxifene and resveratrol through increasing proapoptotic genes. Life Science, 205:45-53.


18. Mothana R. A., Lindequist U., Gruenert R., Bednarski P.J., 2009. Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra. BMC Complementary Medicine, 9:7.


19. Ros B., Le G., McPake B., Fustukian S., 2018. The commercialization of traditional medicine in modern Cambodia. Health Policy and Planning, 33(1):9-16.


20. Si L., Fu J., Liu W., Hayashi T., Mizuno K., Hattori S., Fujisaki H., Onodera S.Ikejima T., 2020. Silibinin-induced mitochondria fission leads to mitophagy, which attenuates silibinin-induced apoptosis in MCF-7 and MDA-MB-231 cells. Archives of Biochemistry and Biophysics, 685:108284.


21. Vitalini S., Madeo M., Tava A., Iriti M., Vallone L., Avato P., Cocuzza C.E., Simonetti P., Argentieri M.P., 2016. Chemical Profile, Antioxidant and Antibacterial Activities of Achillea moschata Wulfen, an Endemic Species from the Alps. Molecules, 21(7):830.


22. Wang Z., Ge S., Li S., Lin H., Lin S., 2020. Anti-obesity effect of trans-cinnamic acid on HepG2 cells and HFD-fed mice. Food and Chemical Toxicology, 137: 111148.


23. Xing J.Y., Song G.P., Deng J.P., Jiang L.Z., Xiong P., Yang B.J., Liu S.S., 2015. Antitumor Effects and Mechanism of Novel Emodin Rhamnoside Derivatives against Human Cancer Cells In Vitro. PLoS One, 10(12): e0144781.


24. Yazdanparast R., Ardestani A., Jamshidi S., 2007. Experimental diabetes treated with Achillea santolina: effect on pancreatic oxidative parameters. Journal of Ethnopharmacology, 112(1):13-18.


25. Zaringhalam J., Akbari A., Tekieh E., Manaheji H., Rezazadeh S., 2010. Achillea santolina reduces serum interleukin-6 level and hyperalgesia during complete Freund's adjuvant-induced inflammation in male Wistar rats. Journal of Chinese Integrative Medicine, 8(12):1180-1189.