The Investigation of 2-Phenoxyethanol on Liver Tissue ofBarbus grypus

Subject Areas : Journal of Animal Biology

نرگس  جوادزاده ¹ (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)
محمدهاشم  تیموری ² (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)
حدیده  معبودی ³ (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)

Received: 2016-03-14 Accepted : 2017-05-21 Published : 2016-01-21

Keywords: Histopathology, Barbus grypus, liver tissue, 2-phenoxyethanol,

Abstract :

This study examined the histopathological effects of anesthetic 2-phenoxy ethanol on liver of Barbus grypus. In this study, a total of 30 Barbus grypus with sizes of 100 ± 2 g were selected. Water temperature with an average of 20°C. Fish were exposed to anesthetic 2-phenoxy ethanol with ascending concentrations of 400, 600 and 700 mg/l for 90 second. Then first stage was"an hour" and the second was "three days after anesthesia" liver biopsy was taken samples were stained at 5-micron sections with hematoxylin – eosin method. Microscopic observations showed the effects of 2-phenoxyethanol on the liver at the dose of 700 mg/l create vacuolization, enlargement offat cells and dilatation of the hepatic sinusoids. At doses of 400 and 600 mg/l, this effect was less.