Evaluation of the Effect of Sedar (Ziziphus spina-christi) Extract on Biochemical Parameters of Skin Lesion in NMRI Mice

Subject Areas : Journal of Animal Biology

Shokooh Sharbatdar ¹ , Nasim Hayati Roodbari ² , Parichehr Yaghmaee ³

1 - Department of Cognitive Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Cognitive Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Cognitive Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2022-08-16 Accepted : 2022-09-09 Published : 2023-08-23

Keywords: Oxidative stress, Cedar, Oserin, Skin Wound Healing,

Abstract :

The skin is one of the largest organs of the body and acts as a protective barrier against external harmful factors including microorganisms. It can be damaged and sometimes if the damage is too much, the hypoderm is also involved, so more time is needed for restoration. The purpose of this research is to investigate cedar plant on skin wound healing in NMRI laboratory mice. 24 mice with a weight of approximately 25-35 grams were bought and after wounding the skin on days 3, 6, 12, 21 they were treated with ointment prepared from cedar and oserin. Mice were randomly divided into three groups: sham, experimental 1, experimental 2. These groups were treated with oserin and Cedar ointment with a dose of 2 mg and 4 mg respectively. Wounds were measured after 3 weeks and their tissue was evaluated by hematoxylin-eosin staining. Biochemical indices of SOD, MDA and TAC were also investigated. Data analysis was done by SPSS version 22 software and Tukey's test. The wound size in Cedar 1 and 2 groups had a significant decrease compared to oserin (p < 0.05). The concentration of MDA in cedar groups 1 and 2 had a significant decrease compared to oserin (p < 0.05). In addition, the activity of SOD and TAC were significantly increased in cedar groups 1 and 2 compared to oserin (p < 0.05). Also, there was a significant increase in the thickness of epidermis and dermis in cedar 1 and 2 compared to oserin (p < 0.05). There was a significant increase in the number of fibroblasts and blood vessels in cedar 1 and 2 compared to oserin (p < 0.05). According to the results of the present study, it can be concluded that cedar extract was effective in wound healing by increasing SOD, TAC and decreasing MDA.

References:

1. Asgarpanah J., Haghighat E. 2012. Phytochemistry and pharmacologic properties of Ziziphus spina christi (L) Willd. African Journal of Pharmacy and Pharmacology, 6(31):2332-2339.


2. Gadanya H.S., Gadanya M.A., Ibrahim I.M., Wudil A.M. 2019. Aqueous leaf extract of Ziziphus Mauritiana is potent on carbon tetrachloride-induced liver damage in albino rats. FUDMA Journal of Sciences, 3(2):124-134.


3. Gorai M., Romdhane R., Maraghni M., Neffati M. 2019. Relationship between leaf gas-exchange characteristics and the performance of Ziziphus spina-christi (L.) Willd. seedlings subjected to salt stress. Photosynthetica, 57(3):897-903.


4. Kim S.Y., Nair M.G. 2019. Macrophages in wound healing: activation and plasticity. Immunology and Cell Biology, 97(3):258-267.


5. Ktari N., Trabelsi I., Bardaa S., Triki M., Bkhairi I., Salem R.B.S. 2017.Antioxidant and hemolytic activities, and effects in rat cutaneous wound healing of a novel polysaccharide from fenugreek (Trigonella foenum-graecum) seeds. International Journal of Biological Macromolecules, 95:625-634.


6. Mardani M., Badiee P., Gharibnavaz M., Jassebi A., Jafarian H., Ghassemi F. 2018. Comparison of anti-Candida activities of the ancient plants Lawsonia inermis and Ziziphus spina christi with antifungal drugs in Candida species isolated from oral cavity. Journal of Conservative Dentistry: JCD, 21(4):359-368.


7. Ma’ruf  T., Dewi P.S., Nurlitasari D.F. 2021. Efficacy of Bidara Leaf (Ziziphus Mauritiana) Viscous Extract to Gingival Wound Healing in Wistar Rats. Journal of International Dental and Medical Research, 14(4):1367-1372.


8. Mazutti da Silva S.M., Rezende Costa C.R, Martins Gelfuso G., Silva Guerra E.N., de Medeiros Nóbrega Y.K., Gomes S.M. 2018. Wound healing effect of essential oil extracted from Eugenia dysenterica DC (Myrtaceae) leaves. Molecules, 24(1):2-18.


9. Polisak B., Kovac V., Milisav I.2021. Antioxidants, food processing and health. Antioxidants, 10(3):433-445.


10. Rahaman M., Hasan M., Eakhlas U., Ivy N., Mofazzal H. 2018. In Vitro Propagation of Zizyphus Jujuba Under Different Hormonal Combinations. Journal of Bangladesh Academy of Agriculture, 8(1):53-59.