Genetic diversity of some wheat landraces of Zanjan province by gliadin patterns

Subject Areas : Agroecology Journal

Arash Mohammadi ¹ (Master of plant breeding Agronomy and Plant Breeding Department College of Agriculture Science Tabriz University Tabriz, Iran)
Mostafa Valizadeh ² (Professor Agronomy and Plant Breeding Department College of Agriculture Science Tabriz University Tabriz, Iran)
Mohammad Moghaddam ³ (Professor Agronomy and Plant Breeding Department College of Agriculture Science Tabriz University Tabriz, Iran)
Yusef Arshad ⁴ (Assistant Professor Seed and Plant Improvement Institute Karaj, Iran Email :)

Received: 2015-01-02 Accepted : 2015-07-26 Published : 2015-08-23

Keywords: Cluster analysis, polymorphism, acid electrophoresis, Nei&#039, s genetic diversity index, seed proteins, electrophoretic patterns,

Abstract :

In present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in 90% of wheats. Also gliadins 12.5 and 15.5 were observed in 86.7% of the wheats. Genetic diversity of the wheats based on total gliadin found to be high (H=0.967). Highest and Lowest genetic variation related to omega-gliadins (H=0.917) and gamma-gliadins (H=0.781) respectively. Wheats of Zanjan showed diversity in terms of gliadin bands number (11-25 bands). The entire wheats, showed specific gliadin pattern. Totally, 29 omega-gliadin patterns, 15 gamma-gliadin patterns, 22 beta-gliadin patterns and 23 alpha-gliadin patterns were observed. Cluster analysis could separate three tetraploid genotypes TN-11372, TN-11734, TN-11743 from hexaploids. These results indicate that gliadin proteins analysis is useful for evaluating of genetic diversity, characterizing of wheat genotypes and separating of different ploidy levels.