Study of phenotypic characteristics of potato bacterial wilt isolates in Fars and Hamedan provinces, Iran

Subject Areas : Agroecology Journal

Javad Razmi ¹ , Nader Hasanzadeh ² , Abolgasem Ghasemi ³ , Asghar Heidari ⁴

1 - Ph. D. Student of Plant Pathology Department, Islamic Azad University of Tehran, Science and Research Branch
2 - Scientific Board of Plant Pathology Department, Islamic Azad University of Tehran, Science and Research Branch
3 - Scientific Boards of Plant Diseases Research Department, Iranian Research Institute of Plant Protection, Tehran
4 - Scientific Board of Plant Diseases Research Department, Iranian Research Institute of Plant Protection, Tehran

Received: 2008-03-01 Accepted : 2008-05-17 Published : 2008-04-20

Keywords: isolate, Potato bacterial wilt, Ralstonia solanacearum, Phenotype,

Abstract :

    In order to study of Phenotypic characteristics of potato bacterial wilt isolates,several potato fields were surveyed for potato wilt plants in Fars and Hamadan provinces of Iran during 2000-2002. The infected samples to potato wilt were collected and screened for bacterial infection under laboratory condition. Isolation and purification of suspected bacterial samples were done on TTC (2,3,5-triphenyl tetrazolium chloride) culture medium.Characteristics of bacterial isolates were determined on the basis of colony morphology, biochemical differentiations, pathogenicity and antibiotic sensitivity tests as well as whole cell protein analysis. In this study, a typical HR symptom was observed in infiltrated tobacco leaves after 24-48 hours. All isolates were assigned in race 3 biovar 2, based on the pathogenicity tests on tomato and potato seedlings and utilization of defined carbohydrates by isolates. Utilization of glucose, sucrose and trehalose were positive for all isolates, whereas it was negative  for ramnose, raffinose, arabinose, fucose and tartrate. H2S production from cysteine, urease, oxidase, catalase and utilizing of sodium citrate were positive, while indole, methyl red, arginine dihydrolyase, gelatin and starch hydrolysis and utilizing of malonate and gluconate were negative. In bacterial cell membrane protein analysis using SDS-PAGE method, no significant differences were observed in electrophoretic patterns of local and standard strains (ACH 0158 from Australia and CIP 10 from Peru) andonly strain RsR79 was a little different from others.

References:

1. باقری، م. و ح. رحیمیان. 1377. تشخیص بیوارهای R. solanacearumعامل پژمردگی سیب‌زمینی در مناطق عمده سیب‌زمینی کاری ایران. خلاصه مقالات سیزدهمین کنگره گیاه‏پزشکی ایران، صفحه 188.


2. بهار، م. و د. دانش. 1367. سبب­‌شناسی پژمردگی سیب‌زمینی در ایران. مجله بیماری‏های گیاهی، جلد 24، صفحات 12-1.
   
   
   1. Buddenhagen, I. W., L. Sequeira and A. Kelman. 1962. Designation of races of Pseudomonas solanacearum. Phytopathology 52: 726.
   
   
   2. Ciampi, L., L. Sequeira and E. R. French. 1980. Latent infection of potato tubers by Pseudomonas solanacearum. American Potato Journal 57:377-386.
   
   
   3. Cowan, S. T. and K. J. Steel. 1965. Manual of the identification of medical bacteria. Cambridge University Press, Cambridge, London.
   
   
   4. Dye, D. W. 1969. A taxonomic study of the genus Erwinia .II. The carotovora groups. New Zealand Journal of Science 12: 81-97.
   
   
   5. Fahy, P. C. and A. C. Hayward. 1983. Media and methods for isolation and diagnostic tests. In: Fahy, P. C. and Persley, G. J. (eds.): Plant bacterial diseases: A diagnostic guide. Pp. 337-378. Academic Press, Sydney, Australia.
   
   
   6. Fahy, P. C. and G. J. Persley. 1983. Plant bacterial diseases: A diagnostic guide. Academic Press, Sydney, Australia, p. 378.  
   
   
   7. French, E. R. 1994. Strategies for integrated control of bacterial wilt of potatoes. In: Hayward, A. C. and Hartman G. L. (eds.): Bacterial Wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB International. Pp.199-207.
   
   
   8. French, E. R., L. Gutarra, P. Aley and J. Elphinstone. 1995. Culture media for Pseudomonas solanacearum isolation, identification and maintenance, Fitopatologia 30: 126- 130.
   
   
   9. Gillings, M. and P. Fahy. 1993. Genomic finger printing and PCR analysis: rapid, sensitive and inexpensive means of differentiating strains of Pseudomonas solanacearum. In: Bacterial Wilt Proceeding of an International Conference held at Kaohsiung, Taiwan, 28-31 Oct. 1992. ACIAR Proceedings 45: 85-92.
   
   
   10. Graham, D. C. and W. Hodgkiss. 1977. Identify of gram negative, yellow pigmented, fermentative bacteria isolated from plants and animals. Journal of Applied Bacteriology 30:175-189.
   
   
   11. Hayward, A. C. 1964. Characteristics of Pseudomonas solanacearum. Journal of Applied Bacteriology 27: 256-277.
   
   
   12. Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology 29: 65- 87.
   
   
   13. Hugh, R. and E. Leifson. 1953. The taxonomic significancance of fermentative versus oxidative metabolism of carbohydrate by various gram negative bacteria. Journal of Bacteriology 66: 22-26.
   
   
   14. Javier, E. Q. 1994. Foreward. In: Hayward, A. C. and Hartman G. L. (eds.):  Bacterial Wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB Interntional. UK. p. XI.
   
   
   15. Kelman, A. 1954. The relationship pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44: 693-695.
   
   
   16. Kelman, A., G. L. Hartman and A. C. Hayward. 1994. Introduction. In: Hayward, A. C. and Hartman G. L. (eds.): Bacterial Wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB Interntional. UK.  p. 1-7.
   
   
   17. Kovacs, N. 1956. Identification of Pseudomonas solanacearum by the oxidase reaction. Nature 178: 703.
   
   
   18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
   
   
   19. Priou, S., P. Aley, E. Chujoy, B. Lemaga and E. French. 1999. Integrated control of bacterial wilt of potato. CIP. Lima, Peru. p. 30.
   
   
   20. Schaad, N. W., J. B. Jones and W. Chen. 2001. Laboratory guide for identification of plant pathogenic bacteria. APS Press. p. 373.
   
   
   21. Suslow, T. V., M. N. Schroth and M. Saka. 1982. Application of a rapid method for gram differentiation of plant pathogenic bacteria and saprophytic bacteria without staining. Phytopathology 72:917-918.
   
   
   22. Thorneley, M. J. 1960. The differentiation of Pseudomonas from other gram negative bacteria on the basis of arginine metabolism. Journal of Applied Bacteriology 13:37-52.
   
   
   23. Yabuuchi, E., Y. Kosako, H. Oyaiza, I. Yano, H. Hotta, Y. Hashimoto, T. Ezaki and M. Arakawa. 1992. Proposal of Burkholderia gen.nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus with type species Burkholderia cepacia comb.nov. Microbiology and Immunology 36:1251-1275.
   
   
   24. Yabuuchi, E., Y. Kosako, I. Yano, H. Hotta and Y. Nisiuchi. 1995. Transfer of two Burkholderia and a Alcaligenes species to Ralstonia gen.nov. : Proposal of Ralstonia picketti comb.nov., Ralstonia solanacearum comb.nov. and Ralstonia eutropha comb.nov. Microbiology and Immunology 39:897-904.