Isolation of native Bacillus thuringiensis Berliner isolates from the agricultural soils of Iran

Subject Areas : Agroecology Journal

Rasoul Marzban ¹ , Golam Reza Salehi Jogzdani ²

1 - Scientific board of Plant Pests and Diseases Research Institute
2 - Scientific board of Biotechnology Institute of Iran

Received: 2005-09-27 Accepted : 2005-12-03 Published : 2006-01-21

Keywords: Iran, Bacillus thuringiensis, isolation, Agricultural soil,

Abstract :

        Isolation and collection of native B. thuringiensis isolates is the base of research on B. thuringiensis. During a 3-year study (1999-2001) 2234 soil samples were collected from 28 provinces of Iran. Bacteria were isolated according modified method of Anwar Hossain et al. Most isolates were isolated from cotton, oil seed, sugar beat and rice field soil. Gilan and Ardabil provinces had been most isolates respectively. A total of 128 crystal- forming isolates were successfully obtained from 28,445 bacterial spore-forming colonies examined. Toxicity tests on Helicoverpa armigera (Lep: Noctoeidea) and Leptinotarsa decemlineata (Col.: Cherisomeliidea) revealed that over %58 of all isolates were toxic.  

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