Antibacterial effect of lavender essential oils on Xanthomonas campestris and Escherichia coli

Subject Areas : Agroecology Journal

Maryam Rabani ¹ , Roya Rezaeian-Doloei ² , Mehdi Jabari-Noghabi ³

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Received: 2014-04-12 Accepted : 2014-08-04 Published : 2014-07-23

Keywords: antibacterial activity, Allelopathy, bactericide, bacteriostatic, Minimum Bactericidal Concentration, Minimum inhibitory concentration,

Abstract :

Using of natural products instead of chemicals and antibiotics to control bacterial pathogens is interest of researchers. The aim of this study was to determine antibacterial effects of lavenderessential oil against Xanthomonas campestris and Escherichia coli in laboratory condition. Chemical compounds of lavender essential oils were identified by GC-MS. Antibacterial activity of essential oils in comparison with gentamicin antibiotic was determined using agar diffusion method. The minimum inhibitory concentration (MIC) of lavenderessential oils was evaluated by macrobroth dilution method by preparing different concentrations of 0.004 to 4% in nutrient broth medium. The minimum bactericidal concentration (MBC) was determined using culture method in nutrient agar medium. Zone of growth inhibition of X. campestris varied from 3.83 ± 0.35 to 18.7 ± 0.77 mm in concentration of 0.25 to 4% respectively and for E. coli in concentration 0.125 to 4 % from 4.5 ± 0.7 to 17.6 ± 0.9 mm. The MIC and MBC of lavenderessential oils against X. campestris equal of 1 and 2% and about E. coli equal to 0.125 and 0.25%, respectively. The most constitutive compounds of lavenderessential oils included of linalool (44.94%) and 1,8-cineol (21.5%). Lavender essential oils can be used as natural antimicrobial agents against pathogenic bacteria.

References:

Aghel N, Moghimipour E, Ameri A (2007) Characterization of an antidermatophyte cream from Zataria multiflora boiss. Iranian Journal of Pharmacological Science 3: 77–84.

2. Agrios GN (1997) Plant Pathology. 4th ed. Academic press. USA.635.

3. Ahmady-abchin  S, Nasrolahi omran A, Jafari N, Mostafapour  MJ, Kia M (2012) Antibacterial effects of Lavandula Stoechas Essential Oil, on Gram Positive and Negative Bacteria In Vitro. Medical Laboratory Journal 6:2.35-41.

4. Akhondzadeh-Basti A, Razavilar V, Misaghi A, Radmehr B, Abbasifar R, Yazdani D ,Akhoundzadeh S ( 2004) Effect of Zataria multiflora Boiss. essential oil on probability of growth initiation of Staphylococcus aureus in a brain heart infusion broth. Journal of Medicinal Plants 3: 53-61.

5. Andrews JM (2001) Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy 48: 5-16.

6. Broomand A, Hamedi M, Emamjomeh Z, Razavi SH, Gholmakani MT (2008) Investigation on the antimicrobial effects of essential oils from dill and coriander seeds on Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella typhimuruim. Iranian Food Science and Technology Research Journal 4: 59-68.

7. Burt S (2004) Essential oils their antibacterial properties and potential applications in food a review. International Journal of Food Microbiology 94(3): 223–253.

8. Conner D.E (1993). Naturally occurring compounds In Davidson PM and Branen AL (Eds). Antimicrobials in Foods 2nd ed. Marcel Dekker Inc. New York 441–467.

9. Dorman D, Deans SG, Noble RC, Surai P (1995) Evaluation in vitro of plant essential oils as natural antioxidants. Journal essential Oil Research 7(6): 645-651.

10. Dufour M, Simmonds RS, Bremer PJ (2003) Development of a method to quantify in vitro the synergistic activity of "natural" antimicrobials. International Journal of Food Microbiology 85(3): 249 - 258.

11. Goran A, Mozafari A, Ghaderi N (2013) Effect of antimicrobial compounds in grapes Vitis.vinifera L. surface sterilized explants in vitro. Congress of Agricultural Research University of Kurdistan 6:1-4.

12. Hasanzadeh N (2005) Technological implication of natural products in plant diseases management with special emphasis on fireblight. Journal of agricultural sciences Islamic Azad University 1:53-67.

13. Hemaiswarya S, Kruthiventi AK, Doble M (2008) Synergism between natural products and antibiotics against infectious diseases. Phytomedici E 15:639–652.

14. Holly RA, Patal D (2005) Improvement in shelf life and safely of perishable food by plant essential oil and smoke antimicrobials. Food Chemistry 22: 273-292.

15. Hoseinzadeh  A, Mohajerfar T, Akhondzadeh basti  A, Khanjari A, Gandomi nasrabadi H, Misaghi A, Sadeghi S (2011) Determination of minimum inhibitory concentration (MIC) of lysozyme and Zataria multiflora Bioss. essential oil on E. coli O157: H7. Journal of Medicinal Plants11:208-217.

16. Karami-Osboo R, Khodaverdi M,  Ali-Akbari F  (2010) Antibacterial Effect of Effective Compounds of Satureja hortensis and Thymus vulgaris Essential Oils against Erwinia amylovora. Journal of Agricultural Science Techology,12(1): 35-45.

17. Kordali S, Kotan R, Mavi A, Cakir A, Ala A, Yildirim A (2005) Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus Artemisia santonicum and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry 53: 9452-9458.

18. Lo Cantore  P, Iacobellis  NS, De Marco A, Capasso  F,  Senatore  F (2004) Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller var. vulgare (Miller) essential oils. Journal of Agricultural and Food Chemistry 52: 7862-7866.

19. Maberley DJ (1997) A portable dictionary of vascular plants.  2^nd ed. Cambridge university press, 210-215.

20. Mohajerfar T, Hoseinzadeh A, Akhondzadeh basti A, Khanjari A, Misaghi A, Gandomi nasrabadi H (2012) Determination of minimum inhibitory concentration (MIC) of Zataria multiflora Bioss. essential oils and lysozim on L. monocytogenes.  Journal of Medicinal Plants 11: 70-78.

21. Omidbeigi R (1995) Approaches to production and processing of medicinal plants. 2^nd ed., Behnashr, Tehran.

22. Oroojalian F, Kasra-Kermanshahi R, Azizi  M, Bassami  MR  ( 2010)  Phytochemical composition of the essential oils from three Apiaceae species and their antibacterial effects on food-borne pathogens. Food Chemistry 120: 765-770.

23. Oorojalian F, Kasra-Kermanshhi R, Azizi M, Basami MR (2009) Synergistic antibacterial activity of the essential oils from three medicinal plants against some important food-borne pathogens by microdilution method. Iranian Journal of Medical and Aromatic Plants 26: 133-146.

24. Ranjbar H, Farzaneh  M,  Sharifii  R, Hadian J,  Mirjalili MH (2008) Classification of selected multi-cut Persian clover germplasm of National Plant Genebank based on agronomic traits. Pajouhesh and Sazandegi 81: 54-60

25. Rasoli A, Rezaie MB (2000). A study on antimicrobial activity and chemical compositions of essential oil from flowers of Lavandula angutifolia and Salvia officinalis. Journal of Kerman University of Medical Sciences 7: 173-181.

26. Rodriguez DJ, Castillo DH, Garcia RR, Sanchez LA (2005)  Antifungal activity of Aloe vera pulp and liquid  fraction against plant pathologenic fungi. Industrial Crops and Products 21:81-87.

27. Smid EJ, Gorris LGM (1999) Natural antimicrobials for food preservation. Handbook of Food Preservation. New York: Marcel Dekker 285–308.

28. Soltandalal MM, Bayat M, Yazdi MH, Aghamiri S, Ghorbanzade-Meshkani M, Peimane- Abedimohtaseb T, Shojai-Saadi B (2012) Evaluation of antimicrobial effect of Zataria multiflora essential oil on antibiotic resistant Staphylococcus aureus isolates from food products. Journal of Kurdistan University of Medical Sciences 17: 21-29.

29. Soylum M, Kurt S, Soylu S (2010) In vitro and in vivo antifungal activities of the essential oil of various plants againsttomato grey mould disease agent Botrytis cinerea. International Journal of Food Microbiology 143:183-189.

30. Ulte A, Bennik MHJ, Moezelaar R (2002) The phenolic hydroxyl group of carvacrol is essential for action against the food pathogen Bacillus cereus. Applied and Environmental Microbiology 68: 1561-1568.