Electrolyte leakage and catalase and peroxidase activity in chickpea genotypes seedling, in response to low temperatures

Subject Areas : Agroecology Journal

Ehsan Fathi ¹ , Iraj Tahmasebi ² , Nasrin Teimoori ³

1 - M.Sc. of Agronomy Faculty of Agriculture Kurdistan University
2 - Assistant professor Department of Agronomy and Plant Breeding Faculty of Agriculture, Kurdistan University
3 - M.Sc. of Agronomy Faculty of Agriculture Kurdistan University

Received: 2015-12-03 Accepted : 2016-05-24 Published : 2016-09-21

Keywords: o Antioxidant enzymes, o Cold stress, o electrolyte leakag, o Azad, o Pirouz, o ILC482,

Abstract :

ABSTRACT     This study was conducted to evaluate the resistance of chickpea genotypes to low temperature stress in seedling growth stage in growth chamber condition. In this experiment temperature levels include -15, -10, -5, 0, 5, 10 and 15 °C applied on five chickpea genotypes such as Arman, Azad, Pirouz, ILC482 and Kaka as factorial based on completely randomized blocks design with three replications. in the of chickpea genotypes With increasing stress cold(Reducing the temperature from 15 to 0 ° C) Level of electrolyte leakage increased and the temperature a 0 ° C reached to maximum This average increase was 21.32%. The temperature range is applied, respectively Azad and ILC482 genotype have the highest and lowest electrolyte leakage were. With decreasing temperature to 0 ° C peroxidase and catalase enzyme activity increased in all genotypes. For temperatures less than 0 ° C genotype was different answers. The highest peroxidase and catalase enzyme activity was related to the genotype ILC482, while genotypes were Azad and pirouz has the lowest enzyme activity. different answers at low temperatures probably due to the influence of of enzymes and other materials that cause reduce damage that reactive oxygen species. Overall, compared to other genotypes ILC482 genotype seems to are the more resistance to cold. According to the results, it is recommended In areas where air temperature in the cultures winter and is waiting to reduce severe The genotypes that are more resistant to cold stress should be used such as ILC482.That Less damage to the In effect of cold on the plant.