Comparison of polymorphism in second intron of bovine Leptin gene in Iranian Taleshi and Holstein cattles using PCR-RFLP

Subject Areas : Agroecology Journal

Ali Reza Dehnad ¹ , Arash Javanmard ² , Fazlollah Afraz ³ , Gorban Elyasi ⁴

1 - Scientific Board, Agricultural Biotechnology Research Center of Northwest and west of Iran
2 - Scientific Board, Agricultural Biotechnology Research Center of Northwest and west of Iran.
3 - Scientific Board of Iranian Research Institute of Animal Science, Karaj
4 - Scientific Board of Research Center of Agriculture and Natural Resources, Azarbayejan e sharqi Province

Received: 2007-07-17 Accepted : 2008-07-26 Published : 2008-10-22

Keywords: PCR-RFLP, cattle, polymorphism, Holstein, Leptin gene, Taleshi,

Abstract :

There are main differences between two Iranian Taleshi and Holstein cattles in milk and meat production and reproduction traits. In this study, polymorphism in second intron of bovine Leptin gene was investigated in order to find molecular mechanisms of these differences in DNA levels. DNA extraction was carried out according to modified salting procedure from blood of one hundered cattles (70 Taleshi and 30 Holstein cattles) and then was amplified 422 bp from second intron of Leptin gene. PCR products were then digested with Sau3AI restriction enzyme for genotype frequency identification. Results revealed that AA, AB and BB genotype frequencies were 61.42, 31.42 and 7.16 percent for Taleshi native cattles and 56.7, 43.3 and zero percent for Holstein cattles, respectively. Frequency of A and B alleles for Taleshi and Holstein cattles were also 77.1, 22.9 and 78.3, 21.7 percent respectively. According the result, polymorphism rate of Iranian cattles in Leptin gene was low and this might cause some breeding difficulties in future.

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