The inhibitory effect of mistletoe extract on the growth and sporulation of Aspergillus flavus in laboratory conditions
Subject Areas : Agroecology Journal
1 - مدیر گروه
Keywords: Aflatoxin, Antimicrobial, Aspergillus flavus, Viscum album, Inhibitor,
Abstract :
Abstract: Nearly 20 to 45% of the world's grains are contaminated with mycotoxins produced by storage fungi. In this study, the antifungal activity of different extracts of the semi-parasitic mistletoe plant on the growth and sporulation of Aspergillus flavus was investigated in laboratory conditions. Different solvents of acetone, ethanol, methanol and water were used to extract extracts of leaves and buds of mistletoe plant. Antifungal activity of extracts was done by Agar Plate Diffusion method. Each of the extracts was added in concentrations of 0, 2, 20, 200 and 2000 microliters per 20 milliliters of culture medium, and the colony growth and sporulation of fungi were evaluated in these mediums. All applied treatments showed a significant effect at the level of 5% on mycelium growth and sporulation of Aspergillus flavus compared to the control. Methanol extract at a concentration of 2000 microliters had the most effect in inhibiting the growth and sporulation of the fungus, and mistletoe aqueous extract showed the least effect. Acetone extract at a concentration of 2000 inhibited sporulation by 99%. From this research, it can be concluded that the methanol extract of mistletoe can be more effective as an antifungal agent in inhibiting the growth and sporulation of Aspergillus flavus than other solvents.