Antimicrobial Effect of Humulus Lupulus, Mentha Longifolia L. and Ocimum Basilicum Hydroalcoholic Extracts On Brucella-Abortus Bacteria Abortusbrucella in Laboratory Conditions
Subject Areas : The Application of Chemistry in Environment
Keywords: MIC, disk diffusion, hydroalcoholic extract, Keywords: Brucellosis, Brucella abertus,
Abstract :
Abstract Brucellosis or maltose fever is caused by intracellular pathogens of brucella, the natural reservoir of which is pets and wildlife. Studies show that medicinal plants can be safely and successfully used in the treatment of bacterial diseases without harmful effects and drug resistance. In this regard, the present study was conducted to treat this disease using medicinal plants. In this study, the inhibitory effect of hydroalcoholic extract of Hazel, Basil and Puneh and double and triple mixture of herbs on growth of Brucella broth in vitro was performed. According to the studies, the above medicinal plants have significant effects on preventing the growth of various pathogenic pathogens. In order to investigate the inhibitory effects of the extracts from the two antibody Biogram and MIC. The results of this study showed that the triple mixture of Puneh, Hazel and Basil extracts with a concentration of 100 mg / ml with a diameter of 28 mm had the greatest diameter of the hole Growth and hydroalcoholic extract of basil with a concentration of 12.5 mg / ml, with a diameter of 5 mm halo, is the smallest diameter of the non-growth zone of the Brucella abertus bacteria. D. Also, the triple mixture of hawk, Pune and Basil showed relatively similar results to rifampin antibiotics. The results of the minimum inhibitory concentration also showed that the least inhibitory concentration of rifampin antibiotic and triple mixture of hydroalcoholic extract of Pune, Hazel and Basil and the most related to the hydroalcoholic extract of Basil's medicinal plant.
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