Evaluation effect of Cycocel foliar application on active substance and some physicochemical characteristics of Cannabis sativa

Subject Areas : Medicinal Plants

ali salehi sardoei ¹ , Hamideh Azad Ghojehbiglou ² , Saeed Fattahi Siah Kamri ³ , Iraj Khazaei ⁴

1 - مربی
2 - MSc. Faculty of Agriculture, Zabol University, Zabol, Iran
3 - MSc. Ferdowsi University of Mashhad, Mashhad, Iran
4 - MSc, Karaj Branch, Islamic Azad University, Karaj, Iran

Received: 2019-01-12 Accepted : 2021-11-14 Published : 2021-12-18

Keywords: Growth inhibitor, Staminate and Pistillate, Tetrahydrocannabind, Biochmical Characteristics,

Abstract :

Cannabis sativa L. is an ancient crop with medicinal and industrial applications that is cultivated to use its fibers and is also an effective painkiller. Cannabinoids are terpenophenolic compounds that have only been identified in cannabis genus. In the present study, the effect of cycocel on the amount of delta-9-tetrahydrocannabinol (THC), the main cannabinoid of cannabis plant, in both male and female cannabis plants was evaluated as a factorial experiment in a completely randomized design with three replications at greenhouse of Bahonar university of Kerman. The main factors included both genders of cannabis plant, male and female and different concentrations of cycocel (0, 500, 1000 and 1500 mg/l). THC was measured by liquid chromatography and UPLC, and total soluble proteins, total soluble sugars and malondialdehyde were analyzed using a spectrophotometer. The results were showed that with increasing cycocel concentration to 500 mg / l, the amount of tetrahydrocannabinol (THC) in the leaves and flowers of the female cannabis significantly increased. Comparing the interaction of genders and cycocel, it was found that the active ingredient of leaves in the female plants had a higher increase than the male plants up to a concentration of 1000 mg/l. The results also demonstrated that concentration of 1000 mg/l had the highest protein content in compare to other treatments in both male and female plants. The content in male gender was higher than the female base up to 1000 mg /l, but at the concentration of 1500 mg/l the female base was better. The lowest quantities of malondialdehyde and other aldehydes was observed in both genders treated with 1000 mg /l cycocel. Overall cycocel concentrations in compare to the control increased the soluble carbohydrates and reducing sugars. According to the obtained results, to achieve the maximum active ingredient of THC, application of 1000 mg/l cycocel in female gender is recommended.

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