Investigating the effect of various chemical and non-chemical treatments break dormancy galbanum seeds Ferula gummosa Boiss.

Subject Areas : Seed technology

mohammadreza Labbafi ¹ , Ali Mehrafarin ² , hasanali naghdibadi ³ , morteza tavakoli ⁴ , majid ghorbani nahogi ⁵

1 - Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran
2 - Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran
3 - Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
4 - Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran
5 - Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran

Received: 2017-07-01 Accepted : 2018-04-30 Published : 2018-08-23

Keywords: Scarification, GA3, Stratification, Ferula gummosa Boiss, Germination percentage and rate,

Abstract :

Ferula gummosa Boiss. is a commercially important medicinal plant that is categorized in endangered herbs list. Seed dormancy and germination is one of the main problems in plant cultivation.This study was conducted to evaluate the effects of chemical, hormonal and thermal treatments on dormancy breaking of galbanum seed, in a completely randomized design (CRD) with 21 treatments in four replications. The percentage and seed germination rate were statistically analyzed. This experiment was done at medicinal plants research center (ACECR) in 2015. Results were showed that the different treatments of seed dormancy breaking had significant (P≤0.01) effect on percentage of seed germination. The highest germination percentage of galbanum seeds observed in combined treatments inclusive of five minutes sulfuric acid (96%) scarification + 30 minutes leaching in water (40˚C) + 7 days imbibition + 40 days stratification + soaking in gibberellic acid (GA3) 1000 ppm. The maximum rate of germination was perceive in combined treatments of 10 minutes sulfuric acid (96%)scarification + 60 minutes leaching in water (40˚C) + 40 days stratification + soaking in GA3 1000 ppm. According to the results,seed germination is improved after sulfuric acid scarification and leaching treatment (physical dormancybreaking), followed by stratification + application of GA3 (morpho-physiological dormancy breaking). Hence, our research offered that galbanum seeds have both physical and morpho-physiological dormancy.

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