The study of quantitative and qualitative changes of Stevia rebaudiana Bertoni. callus under the influence of salicylic acid and salt in solid and liquid culture conditions

Subject Areas : Medicinal Plants

AZIM  GhasemNegad ¹ (Associate Professor, Department of Horticulture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran)
Maral  Salmalian ² (1M.Sc of Medicinal Herbs and Drinks, Gorgan, Iran)
Kambiz  Mashayekhi ³ (Associate Professor, Department of Horticulture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran)

Received: 2017-01-27 Accepted : 2017-05-24 Published : 2017-11-22

Keywords: Tissue culture, Stevia, Photosynthetic pigments, Elicitor, Total and reduced sugar,

Abstract :

Present study was done to evaluate the effect of salinity and salicylic acid in solid and liquid culture media on callus growth and biochemical parameters Stevia like wet and dry weight, photosynthesis pigments, antioxidant activity, total and reduce sugar were measured. A factorial experiment based on completely randomized design with four replications, two different concentrations of salicylic acid (0 and 100µM) and salt (0 and 50mM) in solid and liquid culture media containing 0.5 mg/l NAA and 1 mg/l BA. Result were showed that the using of elicitor and culture and the interaction between the treatments had a significant effect on the measured quantitative and qualitative characteristics. In comparing to the control, the fresh weight callus decreased as affected by salinity and salicylic acid. In contrast, the dry weight of callus significantly increased when treated with elicitors. Internal pigment density of the callus increased when treated by elicitors. The highest antioxidant capacity was observed in liquid medium with containing of 100µM salicylic acid. Total sugar content was observed in medium containing 50 mM salinity. In contrast and compared to the other treatments, the content of reduced sugar in solid culture and under salicylic acid treatment increased significantly. According to the results, duo to increases of metabolit production in Stevia, its need to considering of metabolite culture in invitro conditions and the liquid culture is recommended, while the solid culture would be nessecory to biomass production.

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