Callus induction and essential oil composition of Dracocephalum moldaviaca L.

Subject Areas : Environmental ecology

Syed Mehdi Razavi, ¹ , Ali Reza Ghasemian ² , Sonia Mosavi, ³ , Homa Arshneshin ⁴

1 - 1Associate Professor, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Assistant Professor, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili,
3 - 3M.Sc student, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
4 - M.Sc student, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili,

Received: 2015-11-28 Accepted : 2016-08-15 Published : 2016-09-01

Keywords: Essential oil, Callus, thymol, Dracocephalum moldavica L,

Abstract :

Dracocephalum moldavica L. is an annual aromatic herb, belongs to Lamiaceae family and indigenous of central Asia. In the recent study, we focused on induction of callus tissue and identification of callus essential oil composition. After sterling with ethanol 70% and sodium hypochloride (1%), seeds of the plants were cultured in a Petri dishes line with MS medium. Seedling cotyledons were isolated and transferred to Petri dishes contained MS medium with different combinations of plant hormones, BAP and NAA. For callus formation, the Petri dishes incubated in a growth chamber at 25°C and certain photoperiod of 16/8h light/darkness with 2400 lux light intensity. The produced callus weight was measured for all of treatments for determining optimal hormonal combination. Essential oil compositions of the callus were obtained from the optimal combination by hydro distillation method (Clevenger type apparatus) and were analyzed by gas chromatography-mass spectrometry (GC-MS). The results were showed that callus formation from cotyledons of Dracocephalum moldavica L. seedlings took place in a dose dependent manner of phyto hormones. The maximum Cali genesis was seen in MS medium supplemented with NAA (4 mg/l) and BAP (1.5 mg/l) and thymol (45.99%), decane (26.29%), hexa decanoic acid (7.08%) and dodecane (7.03%) were the main components of callus essential oil.  

References:

1. Ahmad, A., Khan, A., Yousef, S., Khan, L.A. 2010. Proton translocating ATPase mediated fungicidal activity of eugenol and thymol. Fitoterapia, 81(8):1157-62.


2. Andersen, A. 2006. Final report on the safety assessment of sodium p-chloro-m-cresol, p-chloro-m-cresol, chlorothymol, mixed cresols, m-cresol, o-cresol, p-cresol, isopropyl cresols, thymol, o-cymen-5-ol, and carvacrol. International journal of toxicology, 25(1): 29–127.


3. Bajaj, Y.P.S., Furmanowa, M., Olszowska, O. 1988.  Biotechnology of micropropagation of medicinal and aromatic plants. In: Bajaj Y P S (Ed.). Springer-Verlag, New York, 68 p.


4. Buckingham, J. 2005. Dictionary of natural products on CD-ROM. V.13:1. Chapman and Hall/CRC Press, Bocakaton.


5. 5-Filoche, S.K., Soma, K., Sissons, C.H. 2005.  Antimicrobial effects of essential oils in combination with chlorhexidinedi gluconate. Oral Microbiology and Immunology, 20(4): 221–225.


6. Ikeuchi, M., Sugimoto, K., Iwase, A. 2013. Plant callus: Mechanism of induction and repression. The Plant Cell, 25(9): 3159-3173.


7. Maham, M., Akabari, H., Delazar, A.2013. Chemical composition and antinociptive effect of the essential oil of Dracocephalum moldavica L. Pharmaceutical Science, 18(4):187-192.


8. Nesrollahi, P., Razavi, S.M. 2016. Investigation on phytotoxic effects of thymol. 2nd international conference on sustainable development. Strategies and Challenges, 24-26 Feb, Tabriz. Iran.


9. Otroshy, M., Moradi, K. 2012. Effect of explants and growth regulators on direct organogenesis of Dracocephalum kotschyi Boiss. via tissue culture technique. Journal of Herbal Drugs, 3(3): 127-134.


10. Palaniappan, K. and Holley, R. 2010. Use of natural antimicrobials to increase antibiotic susceptibility of drug resistant bacteria. International Journal of Food and Microbiology, 140(2): 164–8.


11. Scragg, A.H.1986. The economics of mass cell culture. In: Morris P, Scragg AH, Stafford A, Fowler MW. (Eds) Secondary metabolism in plant cell cultures. Cambridge University Press, Cambridge, 202p.


12. Sefidkon, F., Jahansoz, M., Davazdahemami, S., Mazaheri, D. 2010. Effects of irrigation water salinity on germination, emergency, biological yield, essence quality and quantity of moldavian balm (Dracocephalum moldavica L.). Plant Production Technology, 2(1): 25-34.


13. Sharifi, A., Hashemi-Sohi, H., Azadi, P., Sharifi, A.A. 2014. Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi. Physiological Molecular Biology of Plants, 20(2):257-262.


14. Trombetta, D., Castelli, F., Sarpietro, M.G., Venuti, V., Cristani, M., Daniele, C., Saija, A.,Mazzanti, G., Bisignano, G. 2005. Mechanisms of Antibacterial Action of Three Monoterpenes. Antimicrobial Agents and Chemotherapy, 49 (6): 2474–8.


15. Yousefzadeh, S., Modarres-Sanavy, S.A., Sefidkon, F., Asgarzadeh, A., ghalavand A. 2010. Effects of different harvest time on essential oil content and composition of dragonhead (Dracocephalum moldavica L.). Iranian Journal of Medicinal and Aromatic Plants, 26 (4): 561-573.