The effect of monolaurin in combination with Mentha pulegium L. and Mentha spicata L. essential oils on Bacillus cereus and E. coli O157:H7: in vitro study

Subject Areas : Veterinary Clinical Pathology

مسلم Neyriz Nagadehi ¹ , S.M Razavi-Rohani ² , گیتی Karim ³ , ودود Razavilar ⁴ , امیر Zeynali ⁵ , رضا Delshad ⁶

1 - Graduat of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Tehran, Iran
2 - Food Hygiene Department of Veterinary Medicine Faculty, Urmia University, Urmia, Iran
3 - Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Tehran, Iran
4 - Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Tehran, Iran
5 - Undergraduate students of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Urmia, Urmia, Iran
6 - Veterinary laboratories Technician

Received: 2009-10-10 Accepted : 2010-05-29 Published : 2010-02-20

Keywords: Monolaurin, Mentha pulegium Essential Oil, Mentha spicata essential oil, E. coli O157:H7, B.cereus,

Abstract :

Monolaurin, pennyroyal (Mentha pulegium L., Labiatae) and spearmint (Mentha spicata L.) essential oils have various antibacterial activities on microorganisms. Enhancement of antibacterial activity and detection of their combined effects on B.cereus ATCC 11778 and E. coli O157:H7 were the purpose of this investigation. Monolaurin preparation, spearmint and pennyroyal essential oils preparation and analysis of their chemical composition with GC-MS method, bacterial inoculums preparation, antimicrobial susceptibility testing using broth micro dilution MIC testing and finally statistic analysis of results with SPSS software package were the material and methods used in this research. MIC (minimum inhibitory concentration) of pennyroyal oil, spearmint oil, monolaurin, monolaurin-pennyroyal oil combination and monolaurin-spearmint oil combination on B.cereus and E. coli O157:H7 were significant (p<0.01). The MIC of monolaurin-pennyroyal oil combination and monolaurin-spearmint oil combination on B.cereus in comparison with the MIC of pennyroyal and spearmint oils separately were significant (p<0.01). Monolaurin spearmint oil combination showed synergistic inhibitory effect on E.coli O157:H7. The most effective antimicrobial agents on B.cereus were monolaurin, monolaurin-spearmint oil combination and monolaurin-pennyroyal oil combination, and the least effective agent was spearmint oil. Also the most effective antimicrobial agent on E. coli O157:H7 was pennyroyal oil, and the least effective agent was monolaurin. Since the MIC of monolaurin-pennyroyal oil combination and monolaurin-spearmint oil combination on E.coli O157:H7 in comparison with the MIC of monolaurin separately were not significant, therefore for significant effect on E.coli O157:H7 and other gram negative bacteria, combination of monolaurin with chelating agents and other natural antimicrobials are suggested.

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