Comparison of antibody titer against the infectious bursal disease virus following the disease with that obtained from live intermediate vaccines using indirect hemagglutination (IHA) test in broiler chicks

Subject Areas : Veterinary Clinical Pathology

عادل Feizi ¹ , مهران Alamdari ² , عبدالله Yadollahi ³ , رضا Azmode ⁴

1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabirz, Iran
2 - Department of Basic Science (Microbiology), Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
3 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
4 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

Received: 2008-10-29 Accepted : 2009-04-20 Published : 2009-02-19

Keywords: broiler chicken, Gumboro, IHA, Vaccine,

Abstract :

       In this study, antibody titer obtained from the outbreak of the infection bursal disease (IBD) was compared with the titer obtained from live intermediate vaccines by indirect haemagglutination (IHA) test in broiler chicks. A total of 450 one day old Cobb chicks were divided into 3 groups each containing 150 chicks and were kept for 42 days in the same rearing conditions. Chicks in groups 1 and 2 received Bursin-2 and D-78 vaccines respectively via drinking water n days 14 and 21. The rest of the chicks were kept as the controls (group 4) and did not receive any vaccine against the IBD. Serum samples were collected from all birds 2 weeks after the second IBD vaccination. Additional 150 serum samples were also collected from 3 broiler flocks that were affected by IBD and had a history of vaccination by the previously mentioned method, two weeks after the last clinical sings were observed (group 3). Antibody titer of the samples against the IBD virus were determined by the IHA test and the results were evaluated using ANOVA and SPSS software. The mean antibody titer obtained from Bursin-2 and D-78 vaccines were 3.19 and 3.21 respectively which is less than the titer of 6 needed for protection against the disease. The antibody titer in affected flocks was 7.19. comparison of the mean titer of the two vaccines did not show any significant difference but there was significant difference between the titer obtained from each vaccine and that of the effected flock (p<0.05). The results indicated that the titer obtained from the vaccines the manner that they were employed did not reach protective levels and there is the possibility of disease. Also the efficacy of IHA test in determining antibody titer against the IBD virus was demonstrated.  

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