Photobiological effects of helium neon laser on hematologic and biochemical factors of rabbit blood
Subject Areas : Veterinary Clinical Pathologyجعفر Rahmani Kahnamoei 1 , Sh Safi 2 , رسول Sadighi bonabi 3 , ایرج Sohrabi Hagdust 4 , M.H Khayat Nouri 5
1 - Graduate of Veterinary Clinical Pathology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science & Research Campus, Tehran
2 - Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
3-Department of Physic, Sanaati Sharif University, Tehran, Iran
3 - Department of Physic, Sanaati Sharif University, Tehran, Iran
4 - Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
5 - Department of Basic Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Keywords: Photobiologic, helium neon laser, hematological factors, Biochemical factors,
Abstract :
Low-level helium neon laser has many applications due to its photobiostimulatory effects. Although the therapeutic effects of low-level laser radiation of different wavelengths and doses are well known, but the exact mechanism of action of the laser radiation on living cells is not yet determined. The present study is designed to evaluate the photobiological effects of 2 mw helium neon laser with wavelength of 632.8 nm on hematologic and biochemical factors of rabbit blood for this purpose, 30 male New Zealand white rabbits with the body weight of 1/5-2 kg were randomly allocated into two groups of control and laser treatment. Animals of both groups were anesthetized and those of laser treatment group were subjected to irradiation with helium neon laser at a wavelength of 632.8 nm and output 2 mw for 30 minutes. Finally blood samples were collected from all animals and the biochemical and hematologic factors evaluated. Significant difference (p<0.05( was observed in the amounts of albumin, total protein, LDH, CPU and WBC between the control and laser treatment group. According to the obtained result, low level laser increases the total protein, albumin, lactase dehydrogenates and creatin kinase activity and decreases which blood cell numbers.
- مجابی ، ع. (1379): بیوشیمی درمانگاهی دامپزشکی، انتشارات دانشگاه تهران، صفحات: 155-154.
- نظیفی، س. (1380): علوم آزمایشگاهی دامپزشکی، آسیب شناسی بالینی، انتشارات دانشگاه شیراز، صفحه: 249.
- Ailioaie, L.M., Ailioaie, C. and Topoliceanu, F. (2001): Laser action in nerve injuries in children, Book of Abstracts, 8th International Congress of EMLA, Moscow, pp: 42.
- Airaksinen, O., Airaksinen, K. and Rantanen, P. (1989): Effects of He-Ne laser irradiation on the trigger points of patients with chronic tension in the neck. Scand. J App. Electrother., 4: 63-65.
- Anders, J., Borke, R. and Woolery, S. (1993): Low-power laser irradiation alters the rate of regeneration of rat facial nerve. Lasers Surg. Med., 13: 72-82.
- Babapour, R. (1995): Low energy laser systems. Clinics in Dermatology Science, 13: 87-90.
- Baxter, G.D. (1994): Therapeutic Lasers. Theory and Practice. Churchill Livingstone, London, pp: 132-139.
- Beckerman, H. and Bouter, L. (1992): The efficacy of laser therapy for musculoskeletal and skin disorders, a criteria-based meta-analysis of randomized clinical trials. Physical Therapy, 72 (7): 483-491.
- Bernard, F., Feldman, J. and Nemi, C. (2000): Schalms Veterinary Hematology. 5th ed., Saunders, London, pp: 455-480.
- Bisht, D., Gupta, S. and Misra, V. (1994): Effect of low intensity laser radiation on healing of open skin wounds in rats. Indian J. Medical Research, 100: 43-46.
- Pretidev, R. (2002): Bioresonance information laser therapy of diabetes mellitus. Low level Laser Therapy, 83: 27-30.
- Ghamsari, S., Taguchi, K. and Abe, N. (1996): Histopathological effect of low-level laser therapy on sutured wounds of the teat in dairy cattle. Veterinary Quarterly, 18(1): 17-21.
- Kapshidze, F. (2001): Optimal dosing of intravascular low-power red laser light as an adjunct to coronary stent implantation: Insights from a porcine coronary stent model. Journal of Clinical Laser Medicine & Surgery, 19(5): 261-265.