Protective effects ofginger (Zingiberofficinale) rhizomeextractonheat-induced testiculardamagein the mouse

Subject Areas : Veterinary Clinical Pathology

bahram amuoghlitabrizi ¹ , mansour khakpour ²

1 - دانشگاه آزاد اسلامی، واحد تبریز، دانشکده دامپزشکی، استادیار گروه علوم درمانگاهی، تبریز، ایران
2 - دانشگاه آزاد اسلامی، واحد تبریز، دانشکده دامپزشکی، استادیار گروه پاتوبیولوژی، تبریز، ایران

Received: 2013-10-06 Accepted : 2014-03-03 Published : 2013-11-22

Keywords: heating, Mice, Testis, : Ginger extract,

Abstract :

Infertility is a complicated problem with medical significance. Gingeras amedicinalherb is used to treata number of diseasessuch assexualweakness. The aim of this study was to evaluate the effects of ginger rhizome extract on heat-induced testicular damage in the mouse. Fourtymale mice were randomly divided into 4 equal groups including: 1- Control, 2- heat stressed, 3 and 4- stressed and treated with ginger extract (1/5 and 3 mg/animal/day). The scrotum of experimental mice was immersed for 20 min in a water bath at 42°C. Control mice were similarly treated except that the water bath was maintained at 23°C. Mice were euthanized after 50 days. Blood samples were collected for analysis of testosterone levels. Testes were removed for histopathological assessment and oxidant/antioxidant status. Heat stress significantly reduced blood testosterone level and increased lipid peroxidation product and decreased antioxidant activities of superoxide dismutase, catalase and glutathione peroxidase (p<0.01). Ginger extract significantly increased blood testosterone level and decreased testis malondialdehyde level and increased antioxidant enzymes activities (p<0.05). Histopathological observations showed progressive degeneration after heating. In the mice treated with ginger extract, testes had normal spermatogenesis and structure.The results indicated that ginger extractimproves spermatogenesis and oxidative damage of testis induced by heat stress in the mouse.

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