Study of changes of some hematological factors of Barbus luteus and its comparison with fishes infected by larval stage of Contracaecum sp.
Subject Areas :
Veterinary Clinical Pathology
alireza golchinmonshadi
1
,
hossein khaj
2
1 - استادیار گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران.
2 - دانشآموخته دانشکده دامپزشکی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران.
Received: 2015-12-19
Accepted : 2016-05-25
Published : 2016-05-21
Keywords:
Parishan lake,
hematological factors,
Barbus luteus,
Contracaecum sp,
Abstract :
Abstract
In order to study the haematological factors of Barbus luteus of Parishan lake and its comparison with fishes infected by larval stage of Contracaecum sp. Sixty fish consisting of 30 healthy and 30 infected by the parasite were caught from the Parishan lake and studied. After blood sampling, autopsy of fishes were performed based on seeking parasitical infection of abdominal cavity to larval stage of Contracaecum sp. Then haematological tests were performed including differential counting of white blood cells, measuring Haematocrit (PCV) and total counting of red blood cells and white blood cells. The result in both groups showed that lymphocytes with 80.56 % had the highest frequency among white blood cells, whereas the frequency of thrombocytes, neutrophils and monocytes were 9.56%, 7.48% and 2.45% respectively, while no basophil and eosinophil were found among the samples. Comparison of white blood cells and thrombocytes among uninfected and infected fishes to the parasites showed that amount of lymphocytes decreased by increasing infection while occurrence of monocytes increased in severe infection in comparison with uninfected stage. Occurrence of neutrophils and thrombocytes increased too. Average amount of haematocrit in uninfected, mild infection and in severe infection fishes were different and decreased. Average counting of red blood cells like haematocrit decreased in uninfected, mild infection and in severe infection, while average counting of white blood cells increased in uninfected, mild infection and in severe infection. Statistically, the changes of lymphocytes, neutrophils, thrombocytes, haematocrit and average counting of white blood cells were significant (p=0.001).
References:
دهقانی، ع. (1384). اکوسیستم تالاب پریشان. چاپ اول، تهران، انتشارات نقش مهر، صفحه: 205.
مجابی، ع. و حیدرنژاد، ا. (1382). خونشناسی دامپزشکی و روشهای آزمایشگاهی. انتشارات مؤسسه آموزش عالی علمی-کاربردی جهاد کشاورزی، صفحات: 95-55.
موحد، ر. (1388). اثر آلودگی انگلی بر برخی از فاکتورهای خونی ماهی سوف سفید دریای خزر (Sander lucioperca). مجله آبزیان و شیلات، دوره 2، شماره 4، صفحات: 72-61.
رشیدی کارسالاری، ز. (1386). بررسی تاثیر آلودگی انگلی بر برخی از فاکتورهای خونی ماهی سفید (Rutilus frissii kutum) در رودخانه تجن. مجموعه خلاصه مقالات اولین کنفرانس ملی علوم شیلات و آبزیان، دانشگاه آزاد اسلامی واحد لاهیجان، لاهیجان، ایران، صفحه: 8.
Ballarin, L., Dalloro, M., Bertotto, D., Libertini, I., Francescon, A. and Barbaro, A. (2004). Hematological parameters in Umbrina cirrosa (Teleostei, Sciaenidae): a comparision between diploid and triploid specimens. Comparative Biochemistry and Physiology, 138: 45-51.
Boon, J.H., Cannaerts, V.H.M., Augustijn, H., Machiels, M.A.M. Decharleroy, D. and Ollevier, F. (1990). The effect of different infection levels with infective Larvae Angullicola crassus. Aquaculture, 87: 243-253.
Bridges A.F. and Manning M.J. (1991). The effects of priming immersions in various human HGG vaccines on humoral and cell mediated immune response after intraperitoneal HGG challenge in the carp. Fish and Shellfish Immunology, 1: 119-129.
Danilo, W.F., Eble, G.J., Kassner, G., Capriaria, F.X., Dafre, A.L. and Ohira, M. (1992). Comparative hematology in marine fish. Comparative Biochemistry and Physiology, 102A: 311-321.
Derzewina, A. (1995). Contribution a l’ttude du tissue lymphoide des ichthyopsidees. Archives de Zoologie Expérimentale et Générale, 3: 145-338.
Ellis, A.E. (1971). The leukocytes of fish: a review. Journal of Fish Biology, 11: 453-491.
Hines, R. and Yashouv, A. (1970). Differential leucocyte counts and total leucocyte and erythrocyte counts for some normal Israeli mirror carp. Bamidgeh, 22: 106-113.
Houston, A.H. and Keen, J.E. (1984). Cadmium inhibition of erythropoiesis in goldfish, Carassius auratus. Canadian Journal of Fisheries and Aquatic Sciences, 41: 1829-1833.
Jamalzadeh, H.R., Keyvan, A., Ghomi, M.R. and Gherardi, F. (2009). Comparison of blood indices in healthy and fungal infected Caspian salmon (Salmo trutta caspius). African Journal of Biotechnology, 8(2): 319-322.
McCarthy, D.H., Stevenson, J.P. and Roberts, M.S. (1973). Some blood parameters of rainbow trout (Salmo gairdneri). Journal of Fish Biology, 5: l-8.
Roberts, R.J. (2001). Fish pathology. 3rd ed., London: Harcourt Publisher Limited, pp: 32-49.
Rowley, A.F. and Ratcliffe, N.A. (1988). Vertebrate Blood Cells. UK: Cambridge, Cambridge University Press, pp: 19-127.
Simmons, A. (1997). Hematology. UK: Simmons, Butterworth‐ Heinemann, pp: 507.
Tavares-Dias, M., Ruas de Moraes, F., Onaka E.M. and Bonadio Rezende, P.C. (2007). Changes in blood parameters of hybrid tambacu fish parasitized by Dolops carvalhoi (Crustacea, Branchiura), a fish louse. Veterinarski Arhiv, 77: 355-363.
Thrall, M.A. (2004). Veterinary Hematology and Clinical Chemistry. USA: Lippincott, Williams and Wilkins, pp: 241, 277-288, 402.
Weinreb, E.L. and Weinreb, S. (1969). A study of experimentally induced endocytosis in a teleost. I. Light microscopy of peripheral blood cell response. Zoologica New York, 54: 25-34.