Seroepidemiological evaluation of Leptospira interrogans strains using by microscopic agglutination test (MAT) in the stray dog population of Qazvin
Mohsen Imandar
1
(
Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Amir Javadi
2
(
Resident of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran,
Iran.
)
Gholamreza Abdollahpour
3
(
Professor, Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
)
Kamran Mirzaie
4
(
Animal and Animal Products-borne Diseases Research Center, Iran Veterinary Organization, Tehran, Iran
)
Parisa Rahimi Siahkal Mahale
5
(
DVM, Board Certificate in Poultry Medicine, The Head of Diagnosis and Treatment unit of Qazvin Province Directorate of Iran Veterinary Organization, Qazvin, Iran
)
Alireza Qanbari
6
(
DVM, Supervisor of the Hygiene Staff of Dashte Bareh Slaughterhouse, Qazvin Provine Directorate of Iran Veterinary Organization, Qazvin, Iran
)
Mostafa Mirzaalimohammadi
7
(
DVM, Graduated in Faculty of Veterinary Medicine, Orumiyeh branch, Islamic Azad University, orumiyeh, Iran
)
Eshagh Taherkhani
8
(
DVM, Expert in Combating Animal Disease, Qazvin Provine Directorate of Iran Veterinary Organization, Qazvin, Iran
)
Javad Sadeghi Alavijeh
9
(
Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
)
Keywords: Dog, Leptospirosis, MAT, Qazvin, Seroepidemiology,
Abstract :
Leptospirosis is an infectious and zoonotic disease with global distribution that is transmitted by contact with the urine of infected animals. Dogs, as one of the important reservoirs of bacteria, cause contamination in humans and domestic animals. The aim of this study was to evaluate seroepidemiological strains of Leptospira interrogans by microscopic agglutination test (MAT) in stray dogs of Qazvin. 74 serum samples were tested for the detection of anti-leptospira antibodies, 15 samples showed a positive reaction with one or two strains of Leptospira interrogans. The results showed that most infected dogs have serum titers of 1:100 (47.1%) and 1:1600 (17.65%). The highest contamination was with Canicula 70.6% and Harjo 11.76%, and Gripotyphosa, Pomona and Icterohemorrhagia strains were in the next positions. Among the positive samples, three samples were related to female dogs and 12 samples were related to male dogs, and the result of Chi square test showed that there is a significant relationship between gender and seropositivity (p<0.05). ). Also, 8 samples belonged to the age groups above 3 years and 7 samples belonged to the age groups less than 3 years, there is no significant relationship between age groups and positive results (p>0.05). Presence of serum contamination among the population of stray dogs in Qazvin can lead to a high prevalence of clinical infection in dogs and domestic and wild animals especially flocks of sheep, which may affect public health due to the zoonotic nature of the disease.
