Serological survey of Influenza A virus infection in horses of some districts of Mahabad city by ELISA method
Subject Areas :
Veterinary Clinical Pathology
Armin Attari
1
,
Arash Araghi-Sooreh
2
1 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 - Associate Professor, Department of Clinical Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran.
Received: 2021-11-02
Accepted : 2022-02-27
Published : 2022-01-21
Keywords:
Elisa,
Mahabad city,
Influenza virus,
Keywords: Horse,
Abstract :
Abstract Equine influenza is an acute, infectious, and highly contagious respiratory disease that is worldwide. This study was aimed to determine the seroprevalence of influenza A virus infection in horses of Mahabad city by competitive ELISA methods. In this study, 200 serum samples were collected from horses in Beytas, Hamzeh abad, Lekben, Kovtar and Dehboker villages located in Mahabad city and then examined by competitive ELISA method. The prevalence of antibodies against the influenza A virus in the study population was 6% )with 95% confidence interval: 2.16-8.7%(. Chi-square test showed no statistically significant relationship between the frequency of positive serum cases of influenza A and the sex of horses )P = 0.354> 0.05(. Horse breeds in the study population included Kurdish, Pony, Working and Arabian horses with 7.6%, 0%, 7.7% and 3.2% positive serum frequency, respectively. Data analysis showed There is no significant difference between the frequency of positive serum cases of influenza A virus and the studied breeds )P = 0.402> 0.05(. This study showed that the serum prevalence of influenza A in the study population is very low. This study is also the first report on the exposure of horses in Mahabad city to the Influenza A virus.
References:
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Nosetto, E., Pecoraro, M., Galosi, M. and Massone, R.)1989(. Isolation of an Equine influenza virus strain and epizootiological study of the 1985-86 outbreak in Argentina revue. Scientifiqueet Technique Office International des Epizooties, 8)1(: 123-128.
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Yin, X., Lu, G., Guo, W., Qi, T., Ma, J., Zhu, C., et al.)2014(. Identification of equine influenza virus infection in Asian wild horses )Equus Przewalskii(. Archives of Virology, 159)5(: 1159-1162.
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Al-Khafaji, M.M. and Hassan, I.Q.) 2016(. Identification of equine influenza A virus antibodies against nonstructural protein (NS1) enables differentiation among infected and vaccinated horses. Mirror of Research in Veterinary Sciences and Animals) MRVSA (, 5)3(: 15-32.
Ataseven, V.S. and Daly, J.M.) 2007(. Seroepidemiology of equine Influenza Virus infection in Turkey. Turkish Journal Veterinary Animal Science, 31)3(: 199-202.
Badiei, K., Pourjafar, M., Samimi, A.S., Ansari-Lari, M., Mohammadi, A. and Ghane, M.) 2013(. Study on risk factors and serologic prevalence of antibodies against Equine Influenza virus in the south of Iran. Comparative Clinical Pathology, 23)4(: 929-932.
Barquero, N., Daly, J.M. and Newton, J.R.) 2007(. Risk factors for influenza infection in 270 vaccinated racehorses: lessons from an outbreak in New market, UK in 2003. Vaccine 25)43(: 7520-7529.
Beuttemmüller, E.A., Woodward, A., Rash, A., dos Santos Ferraz, L.E., Alfieri, A.F., Alfieri, A.A., et al.)2016(. Characterization of the epidemic strain of H3N8 equine influenza virus responsible for outbreaks in South America in 2012. Virology journal, 13)1(: 45-46.
Bererhi, E.H., Kaboula, R., Bouaziz, O., Lakhdara, N. and Dib, A.L.)2012(. Study of Equine Influenza in the region of Khenchela)Algeria(. Agriculture and Biology Journal of North America, 3)4(: 140-144.
Blitvich, B.J., Ibarra-Juarez, L.A., Cortes-Guzman, A.J., Root, J.J., Franklin, A.B. and Sullivan, H.J., et al.)2010 (. Seroprevalence of equine influenza virus in northeast and southern Mexico. Veterinary Record, 166)18(: 565-566.
Boukharta, M., Elharrak, M. and Ennaji, M.)2012(. Seroepidemiological study on equine influenza in Morocco. European Journal of Scientific Research, 68)1(: 147-153.
Barbic, L., Savic, V., Kovacevic, K., Kapetan, J., Stevanovic, V. and Kovac, S. et al.)2018(. Outbreak of equine influenza in Croatia in 2015 and post outbreak epidemiological situation. Veterinarski Fakultet Sveucilista u Zagrebu, 88)4(: 437-451.
Beech, J.)1991(. Infections caused by viruses. In: Equine Respiratory Disorders. Lea & Febiger. Philadelphia, Pennsylvania. Pp: 153-180
Da Silva, A.A., Ranieri, T.M.S., Torres, F.D., Vianna, F.S.L., Paniz, G.R. and Sanseverino, P.B., et al. )2014(. Impact on pregnancies in south Brazil from the influenza A)H1N1( pandemic: cohort study. Pubic Library of Science, 9) 2(: e88624.
Ghadrdan Mashhadi, A.R., Seifi-Abad Shapoori, M.R. and Aghajani, V.)2010(. A serological survey on Equine Influenza in Ahvaz. Veterinary Journal, 23)1(: 59-64. [In Persian]
Hassanpour, A., Amouoghlitabrizi B. and Khakpour M.)2013(. Evaluation of cardiac markers and some serumic biochemical parameters in seropositive horses with equine influenza. Veterinary Clinical Pathology, 7)25(: 1819-1843. [In Persian]
Hassanpour, A., Semsar, P.Y. and Safarmashaei, S.)2012(. Seroprevalence study of Equine Influenza in horses in Tabriz. Annals of Biological Research, 3)12(: 5740-5743.
Hasanpour, A., Vosoughy Irani, A. and Khakpour M.)2014(. Seroprevalence Study of equine Influenza in horses in Ardebil area – Iran. Indian Journal of Fundamental and Applied Life Sciences, 4)3(: 630-633.
Hashemi Mehrjardi, S.H., Pourmahdi Borujeni, M., Ghadrdan Mashhadi, A.R., Siefiabad Shapori, M.R. )2018(. Seroprevalence and risk factors of equine influenza virus infection in horses of Khuzestan province. Veterinary Clinical Pathology, 12)45(: 43-54. [In Persian]
Horner, G. and Ledgard, A.)1988(. A serological survey for Equine Influenza in New Zealand. New Zealand Veterinary Journal, 36)4(: 205-206.
Jurado-Tarifa, E., Daly, J.M., Pérez-Écija, A., Barba-Recreo, M., Mendoza, F.J. and Al-Shuwaikh, A.M., et al. )2018(. Epidemiological survey of equine influenza in Andalusia, Spain. Preventive Veterinary Medicine, 1)151(: 52-56.
Khan, S.U., Gurley, E.S., Gerloff, N., Rahman, M.Z., Simpson, N. and Rahman, M.,et al.)2018(. Avian influenza surveillance in domestic waterfowl and environment of live bird markets in Bangladesh, 2007–2012. Scientific Reports, 8)1(: 1-10.
Lai, A.C., Chambers, T.M., Holland, R.E. Jr., Morley P.S., Haines D.M. and Townsend H.G., et al. )2001(. Diverged evolution of recent equine-2 influenza)H3N8( viruses in the Western Hemisphere. Archive of Virology, 146)6(: 1063-1074.
Minaei, E. and Araghi-Sooreh, A.) 2020(. Assessment of Streptococcus equi infection in apparently healthy working horses of Urmia region by indirect ELISA method. Veterinary Clinical Pathology, 14)55(: 219-227. [In Persian]
Mavadiya, S.V., Raval, S.K., Mehta, S.A., Kanani, A.N., Vagh, A.A. and Tank, P.H., et al.)2012(. Epidemiological survey of equine influenza in horses in India. Revue Scientifique et Technique, 31)3(: 871-875.
Meseko, C.A., Ehizibolo, D.O., Nwokike, E.C. and Wungak, Y.S.)2016(. Serological evidence of equine influenza virus in horse stables in Kaduna, Nigeria. Journal of Equine Science, 27)3(: 99-105.
Nosetto, E., Pecoraro, M., Galosi, M. and Massone, R.)1989(. Isolation of an Equine influenza virus strain and epizootiological study of the 1985-86 outbreak in Argentina revue. Scientifiqueet Technique Office International des Epizooties, 8)1(: 123-128.
Park, A.W., Daly, J.M., Lewis, N.S., Smith, D.J., Wood, J.L.N. and Grenfell, B.T.)2009(. Quantifying the impact of immune escape on transmission dynamics of influenza. Science, 326)5953(: 726-728.
Yin, X., Lu, G., Guo, W., Qi, T., Ma, J., Zhu, C., et al.)2014(. Identification of equine influenza virus infection in Asian wild horses )Equus Przewalskii(. Archives of Virology, 159)5(: 1159-1162.
