The effect of intra-myocardial injection of secretion of bone marrow-derived mesenchymal stem cell on Myocardial infarction in male rabbits

Subject Areas : animal physiology

narges kakadezfuly ¹ , maryam naseroleslami ² , nahid aboutaleb ³

1 - Department of Physiology , Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2 - Department of Cellular and Molecular Biology, Faculty of Advanced Sciences and technology, Tehran Medical Sciences,Islamic azad university, Tehran, Iran
3 - Department of Physiology , Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Received: 2018-11-17 Accepted : 2018-11-25 Published : 2018-05-22

Keywords: Myocardial Infarction, Bone marrow-derived mesenchymal stem cells, Conditioned medium,

Abstract :

Background and Aim: Despite the use of stem cells in the treatment of diseases, especially heart disease, there is a lot of ambiguity about the act of cells and their secretions. Therefore, the purpose of this study was to evaluate the effect of secretion of bone marrow-derived mesenchymal stem cells on myocardial infarction in male rabbits. Materials and Methods: After isolating the stem cells from the bone marrow and preparing the conditioned medium, 30 Male rabbits were randomly divided into three groups(n=10). Myocardial Infarction was induction in two groups and in a group conditioned medium was injected into the hearts of rabbits. Then in the first, fourth and eighth weeks, the heart parameters (HF, EF) were measured by echocardiography and then TNF-α factor was measured in the serum. Heart tissue was removed and the level of angiogenesis and fibrosis were investigated. Results: In the treated group with conditioned medium, the heart parameters (HF, EF) and angiogenesis increased significantly and decrease the level of TNF-α factor and fibrosis was significant in comparison with the Myocardial Infarction group (P<0.05). Discussion: Since the cardiac tissue and its function improved in this research, it seems that the secretion of bone marrow-derived mesenchymal stem cells have therapeutic effects on myocardial infarction.