The application of reverse genetics in plant pathology

Subject Areas : Plant Pests

Jalal Gholamnezhad ¹ (Assistant professor of Agriculture and Natural Resource Faculty, Ardakan University, Ardakan, Iran)

Received: 2015-08-01 Accepted : 2015-12-19 Published : 2015-11-22

Keywords: sequencing, plant pathogen, Reverse genetics,

Abstract :

Reduced cost of genome sequencing of organisms has led to increased number of sequenced genes. But the performance of these genes hasn’t been sequenced yet. However using comparative genetics and bioinformatics has been identified for investigation of sequenced genes function, the interpretation of these genes functions is a challenge. In this regard, sequencing of a large number of fungal plant pathogens and oomycete genomes has been completed. The next major challenge in modern fungal/oomycete biology is to translate the huge amount of genome sequence information into biological functions. Reverse genetics has emerged as a seminal tool for functional genomics investigations. Utilized techniques for reverse genetics like targeted gene disruption/replacement, gene silencing, insertional mutagenesis, and targeting induced local lesions in genomes, will contribute greatly to the understanding of gene function of fungal and oomycete pathogens. This study reviews the reverse genetics approaches which are used to study fungal genomes