Evaluation of the nucleotide and protein sequences of Cytochrome P450 enzymes of the entomopathogenic fungus, Beauveria bassiana
Subject Areas : entomology and othea arthropodsMaryam Rashki 1 , مجتبی Mortezavi 2
1 - Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
2 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
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Abstract :
The nucleotide and protein sequences and phylogenetic relationships between the genes encoding seven cytochrome P450 enzymes for the entomopathogenic fungus Beauveria bassiana strain ATCC90517 existed in the gene bank were assessed. Based on BLASTN results, the highest identity of the seven sequences was observed with the genes of B. bassiana strain ARSEF (84-100%) and then the fungi Cordyceps militaris and Isaria fumosorosea (76-89%), which was according to the BLASTP results. Calculating the percentage of sequence identity with each other using the SIAS program showed that only the amino acid sequences related to CYP52X1 and CYP52G11 genes had more than 47% identity and put in the same family (CYP52). Sequences associated with different families had identity less than 44%. The phylogenetic trees plotted using software IQ-tree v.1.6 based on the nucleotide and amino acid showed that CYP53A26 and CYP617N1 were most closely matched to each otherand put in a separate group. Other P450 genes were also in the same group. Determination of the protein characteristics and motifs of seven enzymes of cytochrome P450 in the fungus B. bassiana is very important and necessary due to their role in iron binding. The presence of various amino acids with different properties in the motifs has been able to increase the host range of this fungus in controlling pest insects.
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