Efficacy of Phyto-synthesized Silver Nanoparticle against Rhopalusiphum padi L. (Hemiptera: Aphididae) and its Carcinogenicity Effect on Mice

Subject Areas : entomology and othea arthropods

S. Imani ¹ (Associate Professor, Department of Entomology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran)
A. Sedighi ² (فارغ التحصیل دکتری حشره شناسی کشاورزی دانشگاه آزاد اسلامی واحد علوم تحقیقات)
G. R. Moshtaghi-Kashanian ³ (دانشیار، استاد دانشکده پزشکی دانشگاه کرمان)
H. Najafi ⁴ (دانشیار عضو هیتت علمی موسسه تحقیقات گیاهپزشکی)
Y. Fathipour ⁵ (استاد، عضو هیات دانشگاه تربیت مدرس دانشکده کشاورزی گروه حشره شناسی کشاورزی)

Received: 2020-04-22 Accepted : 2020-08-23 Published : 2020-08-22

Keywords:

Abstract :

At present, the use of chemical pesticides is the main method of insect control. The use of nano-green technology in the production of new pesticides can be considered and used as a complementary method for pest control. Therefore, in the present study, silver nanoparticles (AgNPs) synthesized from Citrus sinensis orange peel extracts. The synthesized nanoparticles were characterized with UV- visible spectroscopy, X- ray Diffraction, and Transmission Electron Microscopy.The insecticidal effects of silver nanoparticles on Bird cherry-oat aphid, Rhopalusiphum padi L. were conducted under laboratory conditions in a completely randomized design with three replicates of each replication including 20 adult aphids with five concentrations (12.5, 25, 50, 100, 169 ppm) by Filter paper residue. The LC25, LC50 and LC90 of the green synthesized AgNPs against aphids were 8.11 ppm, 30.08 ppm and, 362.61 ppm, respectively. The effects of silver nanoparticles on the liver and kidney tissues of 32 mice were performed in four concentrations (0, 42, 48, 169 ppm) for three months by intraperitoneal injection. Histological examination of the liver and inflammatory cells, hyperaplasia of copper cells, digestion, and degeneration in the liver cells, and increased number of lymphocytes in the kidney tissue were observed. Also, SGOP, SGPT and ALK enzymes increased (p <0.05) in exposure to silver nanoparticles. In general, the results of this study indicate that the use of silver nanoparticles due to its widespread use has adverse environmental effects.