Evaluation of antiviral effects of plant extracts against Tobacco mosaic virus
Subject Areas : Plant Protection
Sasan
GHASEMI
1
()
Saeid
TABEIN
2
()
Keywords:
Abstract :
In the last decades, viral diseases cause to high losses in worldwide crop production. Since, agents with direct effects on virus particles are not available, management approaches of viral diseases includes control of vectors and/or protection of plants according to transformation. According to this, high plant extracts considered as a source antiviral agents. In this study, antiviral effect of methanol extracts of Tagetes patula, Salvia officinalis and Mirabilis jalapa was examined. Collected plants were dried at laboratory condition and then extraction from these samples was carried out by methanol solvent. Antiviral effect of these extracts was screened against tobacco mosaic virus on Nicotiana glutinosa as a local lesion host. Concentration of one twentieth of extracts was used against 2mg/ml purified TMV at three different times: two hours before, simultaneous and two hours after inoculation of virus. After 35 hours post-inoculation, lesions produced by TMV were countered at each treatment. This factorial experiment was performed based on completely randomized design with five replications for each treatment. Data were analyzed and showed two main factors: plant species and time had significant differences at 1% probability level. Means comparison of time treatment showed that use of extracts at simultaneously and after inoculation of virus had most effect on decrease of local lesions. Means comparison of plant species treatment showed that S. officinalis methanol extract had most antiviral effect against TMV. This is a first report of antiviral effect of S. officinalis against phytoviruses.
Awasthi, L. P., Singh, S. P., Verma, H. N., & Kluge, S. 2013. Further studies on the antiviral agent isolated from host palnts, pre-treated with Boerhaavia diffusa glycoprotein. Virology & Mycology 3:1-9.
Azlan, G. J., Marziah, M., Radzali, M. & Johari, R. 2003. Accumulation of Physalin in cells and tissues of Physalis minimal L. III WOCMAP Congress on medicinal and aromatic P
